Phytochemical and biological activities of Bituminaria bituminosa L. (Fabaceae)
| Autor: | Salima Azzouzi, Marie-Geneviève Dijoux-Franca, Nabila Zaabat, Farida Smati, Kamel Medjroubi, Kadour Benlabed, Salah Akkal |
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| Přispěvatelé: | Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Staphylococcus aureus
[SDV]Life Sciences [q-bio] Bituminaria bituminosa L Biology 01 natural sciences Botany medicine Phytochemical composition Escherichia coli Flavone Klebsiella pneumonia ComputingMilieux_MISCELLANEOUS Medicine(all) 010405 organic chemistry Fabaceae General Medicine medicine.disease biology.organism_classification Isoflavone 0104 chemical sciences Antibacterial 010404 medicinal & biomolecular chemistry Phytochemical Bituminaria bituminosa Antioxidant |
| Zdroj: | Asian Pacific Journal of Tropical Biomedicine Asian Pacific Journal of Tropical Biomedicine, 2014, 7 (1), pp.S481-S484. ⟨10.1016/S1995-7645(14)60278-9⟩ |
| ISSN: | 2221-1691 |
| Popis: | ObjectiveTo investigate the phytochemical composition, the antioxidant and antibacterial activities of Bituminaria bituminosa L. (Fabaceae) (B. bituminosa).MethodsThe aerial parts of B. bituminosa yielded two compounds. The structures of these compounds were determinated using UV, 1H-NMR and 13C-NMR experiments and comparison of their spectroscopic properties with literature data. The antibacterial activity of the extracts (CH2Cl2, ethyl acetate and n-BuOH) was determinated using disk diffusion method against standard and clinical strains. Antioxidant potential of n-BuOH extract was evaluated through two methods: DPPH and cupric ion reducing antioxidant capacity assay.ResultsThe n-BuOH extract from B. bituminosa yielded the isolation of isoflavone and flavone. The extracts CH2Cl2, ethyl acetate and n-BuOH demonstrated significant antibacterial activities. CH2Cl2 extract showed the maximum antibacterial activity with high concentration of 2 mg/mL against Staphylococcus aureus ATCC 29213, Klebsiella pneumonia and Escherichia coli ATCC 25922 (20.45 mm, 16.41 mm and 15.74 mm inhibition zone, respectively). The value IC50 was 0.26 μg/mL for n-BuOH extract using DPPH method. Whereas the E% value was 0.10 L/mg every centimeter for cupric ion reducing antioxidant capacity assay.ConclusionsThe phytochemical study of B. bituminosa revealed the presence of isoflavone (daidzin) and flavone (isoorientin) and identified for the first time in this specie. The antibacterial activity of the plant B. bituminosa is certainly related to its chemical content. The n-BuOH extract showed a significant antioxidant activity. |
| Databáze: | OpenAIRE |
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