Single-strand DNA intermediates in phage lambda's Red recombination pathway
| Autor: | Mary M. Stahl, Stuart A. Hill, Franklin W. Stahl |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Recombination
Genetic Multidisciplinary Polarity (international relations) DNA Single-Stranded Biology Biological Sciences Molecular biology DNA-binding protein Bacteriophage lambda In vitro Gene product DNA-Binding Proteins chemistry.chemical_compound Kinetics Viral Proteins chemistry In vivo DNA Viral Recombination DNA In vitro recombination |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 94(7) |
| ISSN: | 0027-8424 |
| Popis: | An assay was developed to assess early intermediates arising in λ’s Red recombination pathway. Double-strand breaks were delivered in vivo to nonreplicating λ chromosomes. Analysis by blot hybridization of total DNA extracts revealed the following: ( i ) long (>1.4 kilobases) single-strand DNA (ssDNA) intermediates; ( ii ) resection proceeding bidirectionally from the break site; ( iii ) single-strand overhangs of 3′ polarity; and ( iv ) in the absence of λ’s ninR functions, a requirement of the red α gene product for the production of ssDNA. Therefore, the physical characteristics exhibited by these ssDNA molecules are consistent with their being an early recombination intermediate in the Red recombination pathway as proposed previously from genetic and in vitro biochemical analyses. |
| Databáze: | OpenAIRE |
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