Application of redox reactions for the determination of valganciclovir hydrochloride in pharmaceuticals
| Autor: | Sameer A. M. Abdulrahman, Nagib A. S. Qarah, Kanakapura Basavaiah |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Detection limit
Prussian blue Valganciclovir Hydrochloride Chromatography Valganciclovir hydrochloride/determination Spectrophotometry/method validation Manganate Permanganate Molar absorptivity 030226 pharmacology & pharmacy 01 natural sciences Redox 0104 chemical sciences RS1-441 Absorbance 010404 medicinal & biomolecular chemistry 03 medical and health sciences chemistry.chemical_compound Pharmacy and materia medica 0302 clinical medicine chemistry Pharmaceutical formulations |
| Zdroj: | Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e17784 Brazilian Journal of Pharmaceutical Sciences; v. 56 (2020); e17784 Brazilian Journal of Pharmaceutical Sciences Universidade de São Paulo (USP) instacron:USP Brazilian Journal of Pharmaceutical Sciences, Vol 56 (2020) Brazilian Journal of Pharmaceutical Sciences, Volume: 56, Article number: e17784, Published: 16 MAR 2020 |
| ISSN: | 2175-9790 1984-8250 |
| Popis: | Two simple, selective and sensitive spectrophotometric methods were developed and validated for the determination of valganciclovir hydrochloride (VLGH) in pure drug and tablets. The first method was based on the reduction of iron(III) to iron(II) by VLGH and subsequent formation of iron(III)-ferricyanide complex (Prussian blue) in acid medium which was measured at 730 nm (method A). In the second method (method B), permanganate was reduced by VLGH to bluish green manganate in alkaline medium and the absorbance was measured at 610 nm. The absorbance measured in each case was related to VLGH concentration. The experimental conditions were carefully studied and optimized. Beer’s law was obeyed over the concentration ranges of 2.5-20.0 and 2.0-40.0 µg mL-1 for method A and method B, respectively, with corresponding molar absorptivity values of 1.28×104 and 6.88×103 L mol-1 cm-1. The limits of detection (LOD) and quantification (LOQ) were 0.11 and 0.33 µg mL-1 (method A) and 0.21 and 0.64 µg mL-1 (method B). Within-day and between-day relative standard deviations (%RSD) at three different concentrations levels were < 2.4%, and the respective relative errors (%RE) were ≤ 3%. The proposed methods were successfully applied to the determination of VLGH in tablets, and the results confirmed that the proposed methods were equally precise and accurate as the official method. |
| Databáze: | OpenAIRE |
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