Calcium Activity Dynamics Correlate with Neuronal Phenotype at a Single Cell Level and in a Threshold-Dependent Manner
| Autor: | Morgan Sehdev, Margaret S. Saha, Peter Kemper, Eileen Ablondi, Atiqur Rahman, Sudip Paudel, Andrew D. Halleran, John P. Marken |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Xenopus neural development lcsh:Chemistry Xenopus laevis 0302 clinical medicine Ca2+ lcsh:QH301-705.5 Cells Cultured Spectroscopy Neurons Neural Plate Chemistry Optical Imaging nervous system Gene Expression Regulation Developmental General Medicine Phenotype Neural stem cell Computer Science Applications Cell biology Female Single-Cell Analysis Neural development Neural plate chemistry.chemical_element embryo Calcium calcium activity Article Catalysis Inorganic Chemistry 03 medical and health sciences Glutamatergic tubb2b SOX2 Animals sox2 Physical and Theoretical Chemistry Molecular Biology development calcium Organic Chemistry 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Cell culture 030217 neurology & neurosurgery |
| Zdroj: | International Journal of Molecular Sciences, Vol 20, Iss 8, p 1880 (2019) International Journal of Molecular Sciences Volume 20 Issue 8 |
| ISSN: | 1422-0067 |
| Popis: | Calcium is a ubiquitous signaling molecule that plays a vital role in many physiological processes. Recent work has shown that calcium activity is especially critical in vertebrate neural development. Here, we investigated if calcium activity and neuronal phenotype are correlated only on a population level or on the level of single cells. Using Xenopus primary cell culture in which individual cells can be unambiguously identified and associated with a molecular phenotype, we correlated calcium activity with neuronal phenotype on the single-cell level. This analysis revealed that, at the neural plate stage, a high frequency of low-amplitude spiking activity correlates with an excitatory, glutamatergic phenotype, while high-amplitude spiking activity correlates with an inhibitory, GABAergic phenotype. Surprisingly, we also found that high-frequency, low-amplitude spiking activity correlates with neural progenitor cells and that differentiating cells exhibit higher spike amplitude. Additional methods of analysis suggested that differentiating marker tubb2b-expressing cells exhibit relatively persistent and predictable calcium activity compared to the irregular activity of neural progenitor cells. Our study highlights the value of using a range of thresholds for analyzing calcium activity data and underscores the importance of employing multiple methods to characterize the often irregular, complex patterns of calcium activity during early neural development. |
| Databáze: | OpenAIRE |
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