Structure elucidation and inhibitory effects on human platelet aggregation of chlorogenic acid from Wrightia tinctoria
| Autor: | Naveen Kunaparaju, Ripal P Amin, Tony Taldone, S. William Zito, Michael A. Barletta, Sunil Kumar |
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| Rok vydání: | 2013 |
| Předmět: |
Chloroform
Chromatography Dose-Response Relationship Drug Molecular Structure Platelet Aggregation biology Plant Extracts Electrospray ionization Ethyl acetate biology.organism_classification Tandem mass spectrometry Apocynaceae Inhibitory Concentration 50 chemistry.chemical_compound Complementary and alternative medicine chemistry Chlorogenic acid Sephadex Humans Wrightia tinctoria Collagen Chlorogenic Acid IC50 Platelet Aggregation Inhibitors |
| Zdroj: | Journal of Complementary and Integrative Medicine. 10 |
| ISSN: | 1553-3840 2194-6329 |
| DOI: | 10.1515/jcim-2012-0048 |
| Popis: | Background Interest in natural compounds as sources of potentially new treatment options is growing rapidly. Preliminary screening of many different plant extracts showed that Wrightia tinctoria acts as a potent human platelet aggregation inhibitor. The aim of the present study was to isolate and characterize the active compound responsible for potent inhibition of human platelet aggregation in vitro. Methods A 70% ethanolic extract derived from W. tinctoria seeds was fractionated with chloroform followed by ethyl acetate. The ethyl acetate fraction was further fractionated and purified through a series of three successive column chromatographic separations using silica gel, Sephadex LH-20, and C-18 columns. Liquid chromatography coupled to negative electrospray ionization tandem mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR) studies were performed in the structure determination of the active phenolic compound present in the ethyl acetate fraction of W. tinctoria seeds. Results A phenolic compound has been isolated and identified as chlorogenic acid by LC-MS/MS and NMR studies. Chlorogenic acid showed concentration-dependent inhibitory effect on collagen-induced platelet aggregation in vitro with an IC50 of 0.2363 μg/μl. Conclusion The present data suggest that chlorogenic acid can be developed as potential antiplatelet agent in the treatment of cardiovascular diseases in diabetes mellitus. |
| Databáze: | OpenAIRE |
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