Bison PRNP genotyping and potential association with Brucella spp. seroprevalence
| ISSN: | 1365-2052 0268-9146 |
|---|---|
| DOI: | 10.1111/j.1365-2052.2005.01240.x |
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::acf2122b3ead9632ef3805c8f3de32c3 https://doi.org/10.1111/j.1365-2052.2005.01240.x |
| Rights: | CLOSED |
| Přírůstkové číslo: | edsair.doi.dedup.....acf2122b3ead9632ef3805c8f3de32c3 |
| Autor: | Peter J. P. Gogan, Christopher M. Seabury, Natalie D. Halbert, James N. Derr, J. W. Templeton |
| Rok vydání: | 2005 |
| Předmět: |
Male
Amyloid Genotype Prions Sequence analysis animal diseases Population Single-nucleotide polymorphism Brucella PRNP Exon Sex Factors Gene Frequency Seroepidemiologic Studies Genetics Animals Protein Precursors education Genotyping education.field_of_study Bison Geography biology Age Factors Sequence Analysis DNA General Medicine biology.organism_classification Antibodies Bacterial Virology United States Female Animal Science and Zoology |
| Zdroj: | Animal Genetics. 36:104-110 |
| ISSN: | 1365-2052 0268-9146 |
| DOI: | 10.1111/j.1365-2052.2005.01240.x |
| Popis: | The implication that host cellular prion protein (PrP(C)) may function as a cell surface receptor and/or portal protein for Brucella abortus in mice prompted an evaluation of nucleotide and amino acid variation within exon 3 of the prion protein gene (PRNP) for six US bison populations. A non-synonymous single nucleotide polymorphism (T50C), resulting in the predicted amino acid replacement M17T (Met --> Thr), was identified in each population. To date, no variation (T50; Met) has been detected at the corresponding exon 3 nucleotide and/or amino acid position for domestic cattle. Notably, 80% (20 of 25) of the Yellowstone National Park bison possessing the C/C genotype were Brucella spp. seropositive, representing a significant (P = 0.021) association between seropositivity and the C/C genotypic class. Moreover, significant differences in the distribution of PRNP exon 3 alleles and genotypes were detected between Yellowstone National Park bison and three bison populations that were either founded from seronegative stock or previously subjected to test-and-slaughter management to eradicate brucellosis. Unlike domestic cattle, no indel polymorphisms were detected within the corresponding regions of the putative bison PRNP promoter, intron 1, octapeptide repeat region or 3'-untranslated region for any population examined. This study provides the first evidence of a potential association between nucleotide variation within PRNP exon 3 and the presence of Brucella spp. antibodies in bison, implicating PrP(C) in the natural resistance of bison to brucellosis infection. |
| Databáze: | OpenAIRE |
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