Genomic and Bioinformatics Analysis of HAdV-4, a Human Adenovirus Causing Acute Respiratory Disease: Implications for Gene Therapy and Vaccine Vector Development
Autor: | John McGraw, Jing Su, Anjan Purkayastha, Donald Seto, Susan E. Ditty, Clark Tibbetts, Ted L. Hadfield |
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Rok vydání: | 2005 |
Předmět: |
Serotype
viruses Molecular Sequence Data Immunology Genomics Genome Viral medicine.disease_cause Microbiology Genome Virus Adenovirus Infections Human Cell Line Tumor Virology Vaccines and Antiviral Agents medicine Humans Vector (molecular biology) Respiratory Tract Infections Phylogeny Genetics biology Adenoviruses Human Computational Biology Outbreak Viral Vaccines Genetic Therapy biology.organism_classification Mastadenovirus Adenoviridae Insect Science DNA Viral |
Zdroj: | Journal of Virology |
ISSN: | 1098-5514 0022-538X |
DOI: | 10.1128/jvi.79.4.2559-2572.2005 |
Popis: | Human adenovirus serotype 4 (HAdV-4) is a reemerging viral pathogenic agent implicated in epidemic outbreaks of acute respiratory disease (ARD). This report presents a genomic and bioinformatics analysis of the prototype 35,990-nucleotide genome (GenBank accession no. AY594253 ). Intriguingly, the genome analysis suggests a closer phylogenetic relationship with the chimpanzee adenoviruses (simian adenoviruses) rather than with other human adenoviruses, suggesting a recent origin of HAdV-4, and therefore species E, through a zoonotic event from chimpanzees to humans. Bioinformatics analysis also suggests a pre-zoonotic recombination event, as well, between species B-like and species C-like simian adenoviruses. These observations may have implications for the current interest in using chimpanzee adenoviruses in the development of vectors for human gene therapy and for DNA-based vaccines. Also, the reemergence, surveillance, and treatment of HAdV-4 as an ARD pathogen is an opportunity to demonstrate the use of genome determination as a tool for viral infectious disease characterization and epidemic outbreak surveillance: for example, rapid and accurate low-pass sequencing and analysis of the genome. In particular, this approach allows the rapid identification and development of unique probes for the differentiation of family, species, serotype, and strain (e.g., pathogen genome signatures) for monitoring epidemic outbreaks of ARD. |
Databáze: | OpenAIRE |
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