Vascular Endothelial Growth Factor–Expressing Mesenchymal Stem Cell Transplantation for the Treatment of Acute Myocardial Infarction
| Autor: | Kazuhide Takeuchi, Yasushi Kitaura, Sakiko Inamoto, Yasuhiro Nakamura, Takashi Omura, Junichi Yoshikawa, Tetsuya Hayashi, Kensuke Ohta, Minoru Yoshiyama, Ryo Matsumoto, Kaname Akioka, Ki-Ryang Koh, Yasukatsu Izumi |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Pathology medicine.medical_specialty Angiogenesis medicine.medical_treatment Genetic enhancement Myocardial Infarction Neovascularization Physiologic Transfection Ventricular Function Left Mesoderm chemistry.chemical_compound Coronary Circulation medicine Animals Humans Myocardial infarction business.industry Growth factor Graft Survival Mesenchymal stem cell Cell Differentiation Stroke Volume Genetic Therapy medicine.disease Capillaries Rats Transplantation Vascular endothelial growth factor Actin Cytoskeleton Microscopy Electron Lac Operon chemistry Rats Inbred Lew Immunology Stem cell Cardiology and Cardiovascular Medicine business Stem Cell Transplantation |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 25:1168-1173 |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/01.atv.0000165696.25680.ce |
| Popis: | Objective— Vascular endothelial growth factor (VEGF) plays an important role in inducing angiogenesis. Mesenchymal stem cells (MSCs) may have potential for differentiation to several types of cells, including myocytes. We hypothesized that transplantation of VEGF-expressing MSCs could effectively treat acute myocardial infarction (MI) by providing enhanced cardioprotection, followed by angiogenic effects in salvaging ischemic myocardium. Methods and Results— The human VEGF 165 gene was transfected to cultured MSCs of Lewis rats using an adenoviral vector. Six million VEGF-transfected and LacZ-transfected MSCs (VEGF group), LacZ-transfected MSCs (control group), or serum-free medium only (medium group) were injected into syngeneic rat hearts 1 hour after left coronary artery occlusion. At 1 week after MI, MSCs were detected by X-gal staining in infarcted region. High expression of VEGF was immunostained in the VEGF group. At 28 days after MI, infarct size, left ventricular dimensions, ejection fraction, E wave/A wave ratio and capillary density of the infarcted region were most improved in the VEGF group, compared with the medium group. Immunohistochemically, α-smooth muscle actin–positive cells were most increased in the VEGF group. Conclusions— This combined strategy of cell transplantation with gene therapy could be a useful therapy for the treatment of acute MI. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|