Excitation under the scanning electron microscope of DNA-associated fluorescence from chicken erythrocyte nuclei, polytene chromosomes and adenovirus 2 virions
| Autor: | Wayne R. McKinney, Paul V. C. Hough |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
In situ
Erythrocytes Histology Base pair Scanning electron microscope Analytical chemistry Biology Chromosomes Fluorescence Pathology and Forensic Medicine law.invention law Microscopy Animals Cell Nucleus Polytene chromosome Adenoviruses Human Resolution (electron density) Virion DNA Microscopy Fluorescence Microscopy Electron Scanning Biophysics Electron microscope Chickens |
| Zdroj: | Journal of Microscopy. 122:193-207 |
| ISSN: | 0022-2720 |
| DOI: | 10.1111/j.1365-2818.1981.tb01259.x |
| Popis: | Biological structures not seen by conventional light microscopy, such as longitudinal striations in polytene chromosomes, and, at the limit of sensitivity, virions of adenovirus 2, have been detected via DNA-associated fluorescence excited under the scanning electron microscope. The maximum sensitivity realized, about 1 detected photon per 700 base pairs, falls short by about an order of magnitude of that required to achieve, in unreplicated specimens, the 2 nm intrinsic resolution of the method. A combination of D2O-H2O substitution with freeze-drying provides the best unquenching procedure found for in situ DNA. DNA-associated fluorescence for light microscopy can be created by moderate exposure of the specimen in the electron microscope. |
| Databáze: | OpenAIRE |
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