New application for expanded porphyrins: sapphyrin and heterosapphyrins as inhibitors of Leishmania parasites
| Autor: | Sara M. Robledo, Iván D. Vélez, David L. Cedeño, Marjorie A. Jones, Victoria H. Nguyen, Jaqueline D. Hooker, Timothy D. Lash, Viviana M. Taylor |
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| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Leishmania Reactive oxygen species Porphyrins biology Singlet oxygen General Medicine biology.organism_classification Biochemistry In vitro Promonocyte Cell Line chemistry.chemical_compound chemistry Animals Humans Physical and Theoretical Chemistry Amastigote Cytotoxicity Intracellular |
| Zdroj: | Photochemistry and photobiology. 88(1) |
| ISSN: | 1751-1097 |
| Popis: | Sapphyrins and a series of related porphyrinoid macrocycles have been investigated as potential agents for the treatment of leishmaniasis. The effectiveness of the compounds was evaluated in vitro upon incubation with Leishmania tarentolae or L. panamensis amastigotes and promastigotes. Their effectiveness was also assessed against intracellular L. panamensis. The cytotoxicity of the compounds was evaluated in vitro using the U937 human promonocyte cell line. Effectiveness and cytotoxicity were assessed in the presence and absence of visible light to assess the photodynamic activity of the compounds. Sapphyrin and two related heterosapphyrins were shown to be particularly effective as inhibitors of Leishmania. A photodynamic effect was observed, which may be attributed to the formation of reactive oxygen species. Yields of singlet oxygen ((1)O(2)) produced were determined in ethanol solutions by direct measurement of (1)O(2) phosphorescence. Confocal microscopy demonstrated that sapphyrin and related macrocycles were taken up by the Leishmania cells and that their presence induces the formation of mitochondrial superoxide. Sapphyrins have been widely investigated as anticancer agents and we here show activity against the Leishmania parasites. |
| Databáze: | OpenAIRE |
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