Reducing NF-κB Signaling Nutritionally is Associated with Expedited Recovery of Skeletal Muscle Function After Damage
| Autor: | George F. Pavis, Francis B. Stephens, Catherine R. Mikus, Benjamin P. Lee, Craig Porter, Nima Alamdari, Doaa R. Abdelrahman, Marlou L. Dirks, Tom S O Jameson, Andrew J. Murton, Benjamin T. Wall |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Endocrinology Diabetes and Metabolism Clinical Biochemistry Muscle Proteins Biochemistry Quadriceps Muscle 0302 clinical medicine Endocrinology muscle damage Medicine Eccentric Ingestion NF-kappa B Healthy Volunteers medicine.anatomical_structure Female Dietary Proteins medicine.symptom Interleukin 1 receptor type I AcademicSubjects/MED00250 Muscle Contraction Signal Transduction medicine.medical_specialty Inflammation Context (language use) Placebo Beverages 03 medical and health sciences Young Adult Internal medicine protein-polyphenol Humans Muscle Skeletal Clinical Research Articles deuterated water business.industry Biochemistry (medical) Skeletal muscle Polyphenols Resistance Training 030229 sport sciences Myalgia Recovery of Function Sports Nutritional Physiological Phenomena 030104 developmental biology inflammation Protein Biosynthesis business Myofibril |
| Zdroj: | The Journal of Clinical Endocrinology and Metabolism |
| ISSN: | 1945-7197 |
| Popis: | Context The early events regulating the remodeling program following skeletal muscle damage are poorly understood. Objective The objective of this study was to determine the association between myofibrillar protein synthesis (myoPS) and nuclear factor-kappa B (NF-κB) signaling by nutritionally accelerating the recovery of muscle function following damage. Design, Setting, Participants, and Interventions Healthy males and females consumed daily postexercise and prebed protein-polyphenol (PP; n = 9; 4 females) or isocaloric maltodextrin placebo (PLA; n = 9; 3 females) drinks (parallel design) 6 days before and 3 days after 300 unilateral eccentric contractions of the quadriceps during complete dietary control. Main Outcome Measures Muscle function was assessed daily, and skeletal muscle biopsies were taken after 24, 27, and 36 hours for measurements of myoPS rates using deuterated water, and gene ontology and NF-κB signaling analysis using a quantitative reverse transcription PCR (RT-qPCR) gene array. Results Eccentric contractions impaired muscle function for 48 hours in PLA intervention, but just for 24 hours in PP intervention (P = 0.047). Eccentric quadricep contractions increased myoPS compared with the control leg during postexercise (24–27 hours; 0.14 ± 0.01 vs 0.11 ± 0.01%·h-1, respectively; P = 0.075) and overnight periods (27–36 hours; 0.10 ± 0.01 vs 0.07 ± 0.01%·h-1, respectively; P = 0.020), but was not further increased by PP drinks (P > 0.05). Protein-polyphenol drinks decreased postexercise and overnight muscle IL1R1 (PLA = 2.8 ± 0.4, PP = 1.1 ± 0.4 and PLA = 1.9 ± 0.4, PP = 0.3 ± 0.4 log2 fold-change, respectively) and IL1RL1 (PLA = 4.9 ± 0.7, PP = 1.6 ± 0.8 and PLA = 3.7 ± 0.6, PP = 0.7 ± 0.7 log2 fold-change, respectively) messenger RNA expression (P < 0.05) and downstream NF-κB signaling compared with PLA. Conclusion Protein-polyphenol drink ingestion likely accelerates recovery of muscle function by attenuating inflammatory NF-κB transcriptional signaling, possibly to reduce aberrant tissue degradation rather than increase myoPS rates. |
| Databáze: | OpenAIRE |
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