Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways
| Autor: | Xiangying Kong, Micun Zhong, Xiao-Hua Pei, Na Lin, Hongye Wan, Ke-Fei Hu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Cellular differentiation Osteoclasts Bone Neoplasms Breast Neoplasms Bone resorption 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Western blot Internal medicine medicine Animals Humans Pharmacology (medical) Receptor Notch1 Receptor Cells Cultured Brucine biology medicine.diagnostic_test Activator (genetics) Macrophages Bone metastasis Cell Differentiation General Medicine Strychnine medicine.disease 030104 developmental biology Endocrinology Complementary and alternative medicine chemistry RANKL 030220 oncology & carcinogenesis biology.protein Cancer research Female Jagged-1 Protein Signal Transduction |
| Zdroj: | Chinese journal of integrative medicine. 23(2) |
| ISSN: | 1672-0415 |
| Popis: | To examine the effects of brucine on the invasion, migration and bone resorption of receptor activator of nuclear factor-kappa B ligand (RANKL)-induced osteoclastogenesis. The osteoclastogenesis model was builded by co-culturing human breast tumor MDA-MB-231 and mouse RAW264.7 macrophages cells. RANKL (50 ng/mL) and macrophage-colony stimulating factor (50 ng/mL) were added to this system, followed by treatment with brucine (0.02, 0.04 and 0.08 mmol/L), or 10 μmol/L zoledronic acid as positive control. The migration and bone resorption were measured by transwell assay and in vitro bone resorption assay. The protein expressions of Jagged1 and Notch1 were investigated by Western blot. The expressions of transforming growth factor-β1 (TGF-β1), nuclear factor-kappa B (NF-κB) and Hes1 were determined by enzyme-linked immunosorbent assay. Compared with the model group, brucine led to a dose-dependent decrease on migration of MDA-MB-231 cells, inhibited RANKL-induced osteoclastogenesis and bone resorption of RAW264.7 cells (P |
| Databáze: | OpenAIRE |
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