Synergistic and antagonistic interactions of anticholinesterase terpenoids in Salvia lavandulaefolia essential oil
| Autor: | Richard M. Wilkins, Sergey U. Savelev, Elaine K. Perry, Edward J. Okello, N.S.L. Perry |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Clinical Biochemistry
Salvia Pharmacognosy Toxicology Biochemistry law.invention Borneol Terpene Behavioral Neuroscience chemistry.chemical_compound Camphor Linalool law Oils Volatile Organic chemistry Drug Interactions Biological Psychiatry Essential oil Pharmacology biology Dose-Response Relationship Drug Terpenes Drug Synergism biology.organism_classification Terpenoid Plant Leaves chemistry Cholinesterase Inhibitors |
| Zdroj: | Pharmacology, biochemistry, and behavior. 75(3) |
| ISSN: | 0091-3057 |
| Popis: | In vitro anticholinesterase activities of eight commercially available terpenoid constituents of Salvia lavandulaefolia have been investigated. These included 1,8-cineole, camphor, alpha-pinene, beta-pinene, borneol, caryophyllene oxide, linalool and bornyl acetate. Dose-dependent inhibition of acetylcholinesterase (AChE) by these chemical constituents was determined using the method of Ellman [Biochem. Pharmacol. 7 (1961) 88]. The IC50 value of 1,8-cineole was 0.06+/-0.01 mg/ml similar to that of the essential oil (0.05+/-0.01 mg/ml). Analyses of the expected inhibitions based on the prediction of a zero interactive response of a combination at its naturally occurring ratios were carried out in comparison with observed inhibition. Minor synergy was apparent in 1,8-cineole/alpha-pinene and 1,8-cineole/caryophyllene oxide combinations, with interaction indexes not exceeding 0.5. In contrast, a combination of camphor and 1,8-cineole was antagonistic with an interaction index of 2. A combination of all eight compounds was zero interactive. A combination of six constituents, excluding 1,8-cineole and camphor, was used to compare the method of expected response of a combination with a method of summation. These findings reveal that the inhibitory activity of the oil results from a complex interaction between its constituents, which produce both synergistic and antagonistic responses between the component terpenes. Understanding such interactions is important in comparing species on the basis of chemical composition. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect