Uncovering the First Atypical DS-1-like G1P[8] Rotavirus Strains That Circulated during Pre-Rotavirus Vaccine Introduction Era in South Africa
| Autor: | Mapaseka L. Seheri, Khuzwayo C. Jere, Valantine N Ndze, Francis E. Dennis, Milton T. Mogotsi, Peter N. Mwangi, Sebotsana Rasebotsa, Martin M. Nyaga, M. Jeffrey Mphahlele |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
viruses Reassortment genome constellation lcsh:Medicine Biology medicine.disease_cause Genome Group A Article whole-genome characterization Rotavirus DS-1 medicine Immunology and Allergy Molecular Biology Gene General Immunology and Microbiology lcsh:R virus diseases Rotavirus vaccine Virology atypical strains Infectious Diseases rotavirus Capsid reassortment |
| Zdroj: | Pathogens Volume 9 Issue 5 Pathogens, Vol 9, Iss 391, p 391 (2020) |
| ISSN: | 2076-0817 |
| DOI: | 10.3390/pathogens9050391 |
| Popis: | Emergence of DS-1-like G1P[8] group A rotavirus (RVA) strains during post-rotavirus vaccination period has recently been reported in several countries. This study demonstrates, for the first time, rare atypical DS-1-like G1P[8] RVA strains that circulated in 2008 during pre-vaccine era in South Africa. Rotavirus positive samples were subjected to whole-genome sequencing. Two G1P[8] strains (RVA/Human-wt/ZAF/UFS-NGS-MRC-DPRU1971/2008/G1P[8] and RVA/Human-wt/ZAF/UFS-NGS-MRC-DPRU1973/2008/G1P[8]) possessed a DS-1-like genome constellation background (I2-R2-C2-M2-A2-N2-T2-E2-H2). The outer VP4 and VP7 capsid genes of the two South African G1P[8] strains had the highest nucleotide (amino acid) nt (aa) identities of 99.6&ndash 99.9% (99.1&ndash 100%) with the VP4 and the VP7 genes of a locally circulating South African strain, RVA/Human-wt/ZAF/MRC-DPRU1039/2008/G1P[8]. All the internal backbone genes (VP1&ndash VP3, VP6, and NSP1-NSP5) had the highest nt (aa) identities with cognate internal genes of another locally circulating South African strain, RVA/Human-wt/ZAF/MRC-DPRU2344/2008/G2P[6]. The two study strains emerged through reassortment mechanism involving locally circulating South African strains, as they were distinctly unrelated to other reported atypical G1P[8] strains. The identification of these G1P[8] double-gene reassortants during the pre-vaccination period strongly supports natural RVA evolutionary mechanisms of the RVA genome. There is a need to maintain long-term whole-genome surveillance to monitor such atypical strains. |
| Databáze: | OpenAIRE |
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