Molecular cloning and analysis of nre, the major nitrogen regulatory gene of Penicillium chrysogenum
| Autor: | Hubertus Haas, B Redl, G Stöffler, George A. Marzluf, Bauer B |
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| Rok vydání: | 1995 |
| Předmět: |
Nitrogen
Genes Fungal Molecular Sequence Data Restriction Mapping Mutant Penicillium chrysogenum Nitrate reductase Aspergillus nidulans Neurospora crassa Fungal Proteins Nitrate Reductases Gene Expression Regulation Fungal Genetics Amino Acid Sequence Cloning Molecular Gene Regulator gene Base Sequence Sequence Homology Amino Acid biology Zinc Fingers Sequence Analysis DNA General Medicine biology.organism_classification Complementation Biochemistry Mutation Transcription Factors |
| Zdroj: | Current Genetics. 27:150-158 |
| ISSN: | 1432-0983 0172-8083 |
| DOI: | 10.1007/bf00313429 |
| Popis: | We have isolated the Penicillium chrysogenum nre gene which is homologous to the major nitrogen regulatory genes areA from Aspergillus nidulans and nit-2 from Neurospora crassa. Overall, nre shows 60% identity to areA and 30% identity to nit-2 at the amino-acid level. The gene encodes a protein of 835 amino-acid residues and contains a single Cys2/Cys2-type zinc finger with an adjacent basic region and a putative acidic activation region. In the DNA-binding domain, 98% of the amino-acid residues are identical in nre, areA and nit-2. The nre gene has been shown to be functional in N. crassa by heterologous complementation of a nit-2 mutant. Growth tests indicated that transformants could utilize nitrate, amino-acids, purines and amides as sole nitrogen sources. Nitrate reductase activity assays performed with transformants demonstrated that nitrogen control was completely normal. Complementation of N. crassa nit-2 mutants with 5'-deletion clones of nre suggests the possible presence of an internal promoter within the coding region. Northern analysis and ribonuclease protection assays of total cellular RNA indicated that nre encodes a 3.2-kb transcript which is reduced in content under conditions of nitrogen repression. |
| Databáze: | OpenAIRE |
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