Composition and Dynamics of Bacterial Communities of a Drinking Water Supply System as Assessed by RNA- and DNA-Based 16S rRNA Gene Fingerprinting
| Autor: | Manfred G. Höfle, Richard Christen, Petra Westphal, Claudia Höltje, Arndt Mehling, Ingrid Brettar, Julia Bötel, Stefan Eichler |
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| Přispěvatelé: | Department of Environmental Microbiology, GBF-German Research Center for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany., Institut de signalisation, biologie du développement et cancer (ISBDC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Department of Vaccinology, Helmholtz Centre for Infection Research (HZI) |
| Rok vydání: | 2006 |
| Předmět: |
MESH: Sequence Analysis
DNA Fresh Water Public Health Microbiology 010501 environmental sciences 01 natural sciences Applied Microbiology and Biotechnology RNA Ribosomal 16S MESH: DNA Fingerprinting MESH: Ecosystem Food science MESH: Water Supply [SDV.BDD]Life Sciences [q-bio]/Development Biology Polymorphism Single-Stranded Conformational Betaproteobacteria 0303 health sciences MESH: DNA Ribosomal Ecology biology Community structure 6. Clean water MESH: RNA Ribosomal 16S DNA profiling MESH: Fresh Water MESH: Drinking Biotechnology DNA Bacterial Molecular Sequence Data Drinking DNA Ribosomal 03 medical and health sciences Tap water Water Supply MESH: Genes rRNA Raw water Ribosomal DNA Ecosystem 0105 earth and related environmental sciences MESH: Polymorphism Single-Stranded Conformational MESH: Molecular Sequence Data Bacteria 030306 microbiology Bacteroidetes Genes rRNA Sequence Analysis DNA Ribosomal RNA biology.organism_classification MESH: DNA Bacterial DNA Fingerprinting MESH: Bacteria Food Science |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2006, 72 (3), pp.1858-72. ⟨10.1128/AEM.72.3.1858-1872.2006⟩ |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.72.3.1858-1872.2006 |
| Popis: | Bacterial community dynamics of a whole drinking water supply system (DWSS) were studied from source to tap. Raw water for this DWSS is provided by two reservoirs with different water characteristics in the Harz mountains of Northern Germany. Samples were taken after different steps of treatment of raw water (i.e., flocculation, sand filtration, and chlorination) and at different points along the supply system to the tap. RNA and DNA were extracted from the sampled water. The 16S rRNA or its genes were partially amplified by reverse transcription-PCR or PCR and analyzed by single-strand conformation polymorphism community fingerprints. The bacterial community structures of the raw water samples from the two reservoirs were very different, but no major changes of these structures occurred after flocculation and sand filtration. Chlorination of the processed raw water strongly affected bacterial community structure, as reflected by the RNA-based fingerprints. This effect was less pronounced for the DNA-based fingerprints. After chlorination, the bacterial community remained rather constant from the storage containers to the tap. Furthermore, the community structure of the tap water did not change substantially for several months. Community composition was assessed by sequencing of abundant bands and phylogenetic analysis of the sequences obtained. The taxonomic compositions of the bacterial communities from both reservoirs were very different at the species level due to their different limnologies. On the other hand, major taxonomic groups, well known to occur in freshwater, such as Alphaproteobacteria , Betaproteobacteria , and Bacteroidetes , were found in both reservoirs. Significant differences in the detection of the major groups were observed between DNA-based and RNA-based fingerprints irrespective of the reservoir. Chlorination of the drinking water seemed to promote growth of nitrifying bacteria. Detailed analysis of the community dynamics of the whole DWSS revealed a significant influence of both source waters on the overall composition of the drinking water microflora and demonstrated the relevance of the raw water microflora for the drinking water microflora provided to the end user. |
| Databáze: | OpenAIRE |
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