Two Auxin Response Elements Fine-Tune PINOID Expression During Gynoecium Development in Arabidopsis thaliana
| Autor: | William Tasker-Brown, Bethany Runciman, Lars Østergaard, André Kuhn |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
auxin response lcsh:QR1-502 Arabidopsis Regulator cis-regulation Context (language use) Flowers Protein Serine-Threonine Kinases Response Elements 01 natural sciences Biochemistry lcsh:Microbiology Article 03 medical and health sciences Gene Expression Regulation Plant Drosophila Proteins Arabidopsis thaliana gynoecium PINOID Promoter Regions Genetic Molecular Biology Gene Transcription factor 030304 developmental biology Homeodomain Proteins Regulation of gene expression ETTIN 0303 health sciences Indoleacetic Acids biology Arabidopsis Proteins INDEHISCENT Gene Expression Profiling fungi food and beverages biology.organism_classification Phenotype Cell biology Regulatory sequence Mutation plant development gene regulation 010606 plant biology & botany |
| Zdroj: | Biomolecules Volume 9 Issue 10 Biomolecules, Vol 9, Iss 10, p 526 (2019) |
| ISSN: | 2218-273X |
| DOI: | 10.3390/biom9100526 |
| Popis: | The plant hormone auxin controls almost all aspects of plant development through the gene regulatory properties of auxin response factors (ARFs) which bind so-called auxin responsive elements (AuxREs) in regulatory regions of their target genes. It has been proposed that ARFs interact and cooperate with other transcription factors (TFs) to bind to complex DNA-binding sites harboring cis-elements for several TFs. Complex DNA-binding sites have not been studied systematically for ARF target genes. ETTIN (ETT ARF3) is a key regulator of gynoecium development. Cooperatively with its interacting partner INDEHISCENT (IND), ETT regulates PINOID (PID), a gene involved in the regulation gynoecium apical development (style development). Here, we mutated two ETT-bound AuxREs within the PID promoter and observed increased style length in gynoecia of plants carrying mutated promoter variants. Furthermore, mutating the AuxREs led to ectopic repression of PID in one developmental context while leading to ectopically upregulated PID expression in another stage. Our data also show that IND associates with the PID promoter in an auxin-sensitive manner. In summary, we demonstrate that targeted mutations of cis-regulatory elements can be used to dissect the importance of single cis-regulatory elements within complex regulatory regions supporting the importance of the ETT-IND interaction for PID regulation. At the same time, our work also highlights the challenges of such studies, as gene regulation is highly robust, and mutations within gene regulatory regions may only display subtle phenotypes. |
| Databáze: | OpenAIRE |
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