Pear metal transport protein PbMTP8.1 confers manganese tolerance when expressed in yeast and Arabidopsis thaliana

Autor: Wei Zhang, Ye Fan, Dongfang Gu, Lu Zheng, Yuerong Wang, Shaoling Zhang, Yurou Ma, Jiyu Li, Xi Chen, Yaping Lu
Rok vydání: 2020
Předmět:
Health
Toxicology and Mutagenesis
Mutant
Saccharomyces cerevisiae
0211 other engineering and technologies
Adaptation
Biological
Arabidopsis
02 engineering and technology
Vacuole
010501 environmental sciences
Manganese transporter
01 natural sciences
Plant Roots
Environmental pollution
Pyrus
Pre-vacuolar compartment
Plant Cells
Arabidopsis thaliana
GE1-350
Cation Transport Proteins
0105 earth and related environmental sciences
021110 strategic
defence & security studies
Manganese
biology
Chemistry
fungi
Public Health
Environmental and Occupational Health
food and beverages
General Medicine
Subcellular localization
biology.organism_classification
Plant cell
Pollution
Yeast
Environmental sciences
Plant Leaves
Ion homeostasis
TD172-193.5
Biochemistry
PbMTP8.1
Vacuoles
Environmental Pollutants
CDF family
Zdroj: Ecotoxicology and Environmental Safety, Vol 208, Iss, Pp 111687-(2021)
ISSN: 1090-2414
Popis: Manganese (Mn) is demonstrated to be essential for plants. Ion homeostasis is maintained in plant cells by specialized transporters. PbMTP8.1, which encodes a putative Mn-CDF transporter in Pyrus bretschneideri Rehd, was expressed mainly in leaves and complemented the Mn hypersensitivity of the Mn-sensitive yeast mutant △pmr1 in previous research conducted by our laboratory. In the present study, we report that the expression of PbMTP8.1 can enhance Mn tolerance and accumulation in Saccharomyces cerevisiae. Subcellular localization analysis of the PbMTP8.1-GFP fusion protein indicated that PbMTP8.1 was targeted to the pre-vacuolar compartment (PVC). In addition, the overexpression of PbMTP8.1 in Arabidopsis thaliana conferred increased resistance to plants under toxic Mn levels, as indicated by increased fresh and dry weights of shoots and roots. Mn accumulation in vacuoles of PbMTP8.1-overexpressing plants was significantly increased when compared with that in wild-type plants under Mn stress. This suggests that a considerable proportion of Mn enters into the vacuoles through a PbMTP8.1-dependent mechanism. Taken together, these results indicate PbMTP8.1 is a Mn-specific transporter that is localized to the PVC, and confers Mn tolerance by sequestering Mn into the vacuole.
Databáze: OpenAIRE
Externí odkaz: