Crassaminicella profunda gen. nov., sp. nov., an anaerobic marine bacterium isolated from deep-sea sediments
| Autor: | Grégoire Galès, Raja Lakhal, Marie-Laure Fardeau, Nathalie Pradel, Anne Postec, Anne Godfroy, Jean-Luc Cayol, Bernard Ollivier |
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| Přispěvatelé: | Institut méditerranéen d'océanologie (MIO), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Toulon (UTLN), Laboratoire de Microbiologie et Biotechnologie des Environnements Chauds, Université de la Méditerranée - Aix-Marseille 2-Université de Provence - Aix-Marseille 1, Laboratoire de microbiologie des environnements extrêmophiles (LM2E), Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2015 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Biology Gram-Positive Bacteria Microbiology Ferric Compounds California Clostridia 03 medical and health sciences chemistry.chemical_compound Bacteria Anaerobic Phylogenetics RNA Ribosomal 16S Mexico Ecology Evolution Behavior and Systematics ComputingMilieux_MISCELLANEOUS Phospholipids Phylogeny 030304 developmental biology chemistry.chemical_classification Thiosulfate 0303 health sciences Base Composition Strain (chemistry) 030306 microbiology Sulfates Fatty Acids General Medicine Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA Amino acid Bacterial Typing Techniques chemistry Biochemistry [SDE]Environmental Sciences Fermentation [SDE.BE]Environmental Sciences/Biodiversity and Ecology Bacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2015, 65 (9), pp.3097-3102. ⟨10.1099/ijsem.0.000386⟩ International Journal Of Systematic And Evolutionary Microbiology (1466-5026) (Soc General Microbiology), 2015-09, Vol. 65, P. 3097-3102 International Journal of Systematic and Evolutionary Microbiology, 2015, 65 (9), pp.3097-3102. ⟨10.1099/ijsem.0.000386⟩ |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | A novel, anaerobic, chemo-organotrophic bacterium, designated strain Ra1766HT, was isolated from sediments of the Guaymas basin (Gulf of California, Mexico) taken from a depth of 2002 m. Cells were thin, motile, Gram-stain-positive, flexible rods forming terminal endospores. Strain Ra1766HT grew at temperatures of 25–45 °C (optimum 30 °C), pH 6.7–8.1 (optimum 7.5) and in a salinity of 5–60 g l− 1 NaCl (optimum 30 g l− 1). It was an obligate heterotrophic bacterium fermenting carbohydrates (glucose and mannose) and organic acids (pyruvate and succinate). Casamino acids and amino acids (glutamate, aspartate and glycine) were also fermented. The main end products from glucose fermentation were acetate, butyrate, ethanol, H2 and CO2. Sulfate, sulfite, thiosulfate, elemental sulfur, fumarate, nitrate, nitrite and Fe(III) were not used as terminal electron acceptors. The predominant cellular fatty acids were C14 : 0, C16 : 1ω7, C16 : 1ω7 DMA and C16 : 0. The main polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phospholipids. The G+C content of the genomic DNA was 33.7 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain Ra1766HT was affiliated to cluster XI of the order Clostridiales, phylum Firmicutes. The closest phylogenetic relative of Ra1766HT was Geosporobacter subterraneus (94.2 % 16S rRNA gene sequence similarity). On the basis of phylogenetic inference and phenotypic properties, strain Ra1766HT ( = DSM 27501T = JCM 19377T) is proposed to be the type strain of a novel species of a novel genus, named Crassaminicella profunda. |
| Databáze: | OpenAIRE |
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