Low detection rate and maternal provenance of hepatitis B virus S gene mutants in cases of failed postnatal immunoprophylaxis in England and Wales
| Autor: | J. Heptonstall, R. P. Eglin, S. O'Connell, Siew Lin Ngui, C. G. Teo |
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| Rok vydání: | 1997 |
| Předmět: |
HBsAg
Molecular Sequence Data Immunoglobulins medicine.disease_cause Polymerase Chain Reaction Virus law.invention Orthohepadnavirus law Pregnancy medicine Immunology and Allergy Missense mutation Humans Amino Acid Sequence Codon Gene Polymerase chain reaction Hepatitis B virus Hepatitis B Surface Antigens biology Immunization Passive Infant Newborn Infant biology.organism_classification Hepatitis B Virology Infectious Disease Transmission Vertical Infectious Diseases Hepadnaviridae Mutation Female |
| Zdroj: | The Journal of infectious diseases. 176(5) |
| ISSN: | 0022-1899 |
| Popis: | Hepatitis B virus (HBV) infection occurred despite full passive-active immunoprophylaxis in 20 of 321 infants born to mothers seropositive for hepatitis B e antigen. In 2 (12%) of 17 infected infants, mother-infant DNA sequence mismatches were found in a segment of the HBV S gene coding for antigenic determinants of the HBV surface antigen (HBsAg) amplified from sera by polymerase chain reaction (PCR). Point substitutions occurred in codons 120, 134, and 144 of the HBsAg polypeptide in the variant sequence of 1 infant and in codon 126 in the other; all were missense mutations. Mutant sequences could not be recovered from maternal sera by PCR cloning but were selectively generated using an amplification refractory mutation system. The frequency of potential vaccine escape mutants is therefore low, and these preexist maternally as minor variants. |
| Databáze: | OpenAIRE |
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