An in vitro focus-induction assay for xenotropic murine leukemia virus, feline leukemia virus C, and the feline--primate viruses RD-114/CCC/M-7
| Autor: | Paul T. Peebles |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Virus Cultivation
viruses Virus Replication Feline leukemia virus Virus Cell Line Sarcoma Viruses Murine Virology biology.animal Murine leukemia virus Bioassay Animals Mink Hexadimethrine Bromide biology Leukemia Virus Feline DEAE-Dextran Defective Viruses biology.organism_classification Molecular biology In vitro Leukemia Virus Murine Retroviridae Cell culture Moloney murine sarcoma virus |
| Zdroj: | Virology. 67(1) |
| ISSN: | 0042-6822 |
| Popis: | A rapid focus-induction assay has been developed for certain replicating (r+) but nontransforming (t−) mammalian type C viruses including xenotropic murine leukemia virus (X-MuLV), feline leukemia virus C (FeLV-C), and feline-primate viruses RD-114/CCC/M-7. Mink cells infected only with r−t+ defective Moloney murine sarcoma virus (MSV) were established into a phenotypically flat cell line (S+L− MiCl1). When S+L− MiCl1 monolayers were superinfected with dilutions of the r+t− type C viruses, foci formed with one-hit kinetics. Focus induction was enhanced by treatment of the cells with either DEAE-dextran or polybrene. Quantitation of r+t− virus by use of S+L− MiCl1 cells was comparable to that of other biological assay systems. |
| Databáze: | OpenAIRE |
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