Rutin ameliorates kidney interstitial fibrosis in rats with obstructive nephropathy

Autor: Ding Liu, Li-pei Fan, Hua Chen, Ying Guo, Bin Wang, Qiu-hua Zhu, Liang-sheng Yue, Liuyang Li, Min Li, Ming Zhao
Rok vydání: 2016
Předmět:
Male
0301 basic medicine
Rutin
Anti-Inflammatory Agents
Kidney
urologic and male genital diseases
chemistry.chemical_compound
0302 clinical medicine
Cell Movement
Fibrosis
Immunology and Allergy
Cells
Cultured
biology
Extracellular Matrix
medicine.anatomical_structure
030220 oncology & carcinogenesis
Cytokines
Kidney Diseases
Inflammation Mediators
medicine.symptom
Signal Transduction
Ureteral Obstruction
medicine.medical_specialty
Epithelial-Mesenchymal Transition
Immunology
Inflammation
Proinflammatory cytokine
Transforming Growth Factor beta1
03 medical and health sciences
Internal medicine
medicine
Animals
Smad3 Protein
Rats
Wistar
Pharmacology
business.industry
Macrophages
medicine.disease
Obstructive Nephropathy
Rats
Fibronectin
030104 developmental biology
Endocrinology
chemistry
biology.protein
Tubulointerstitial fibrosis
business
Zdroj: International Immunopharmacology. 35:77-84
ISSN: 1567-5769
DOI: 10.1016/j.intimp.2016.03.029
Popis: Rutin reportedly conveys many beneficial effects, including renoprotection; however, it has not yet been demonstrated to have a renoprotective effect against obstructive nephropathy. The present study is the first to show a protective effect of rutin against obstructive renal injury induced by unilateral ureteral obstruction (UUO). A total of 24 male Wistar rats were randomly divided into four groups of six rats each, including vehicle- or rutin-treated sham operated groups, and vehicle- or rutin-treated UUO groups. Rats received daily oral gavage of rutin (100mg/kg) for 2weeks. All rats were euthanized on postoperative day 14. Histological findings showed that rutin administration significantly reduced renal interstitial injury and suppressed interstitial collagen deposits in UUO rats. Moreover, rutin decreased macrophage infiltration, proinflammatory cytokine expression and phosphorylation of nuclear factor-κB p65. Furthermore, rutin inhibited extracellular matrix accumulation by reducing expression of type I/III collagen and fibronectin. Rutin also prevented the epithelial-mesenchymal transition processes of renal tubular cells by decreasing α-smooth muscle actin expression and retaining E-cadherin expression. These effects of rutin were in parallel with the reductions in Smad3 activity and pivotal to the fibrogenic potential of TGF-β1. Taken together, the renoprotective effects of rutin in obstructive nephropathy were likely due to anti-inflammatory effects and inhibition of TGF-β1/Smad3 signaling.
Databáze: OpenAIRE
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