Optimized cryopreservation of mixed microbial communities for conserved functionality and diversity
| Autor: | Ramiro Vilchez-Vargas, Peter Vandamme, Nico Boon, Siegfried E. Vlaeminck, Sven Hoefman, Emilie Courtens, Annelies Geirnaert, Kim Heylen, Adrian Ho, Dietmar H. Pieper, Ruy Jauregui, Frederiek-Maarten Kerckhof, Tom Van de Wiele |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
METHANOTROPHIC COMMUNITIES
HETEROTROPHIC GROUNDWATER COMMUNITY Denitrification IMPACT Applied Microbiology lcsh:Medicine Tryptic soy broth Cryopreservation chemistry.chemical_compound Feces METHANE Cryoprotective Agents Food science lcsh:Science LONG-TERM STORAGE Phylogeny Autotrophic Processes Multidisciplinary Microbial Viability Ecology Microbiota ROTATING BIOLOGICAL CONTACTOR Community Ecology Anammox Anaerobic bacteria Research Article Biotechnology Biology Euryarchaeota Microbiology Microbial Ecology Cryobiology Environmental Biotechnology Humans PRESERVATION Community Structure Probiotics lcsh:R Ecology and Environmental Sciences Biology and Life Sciences Species Interactions chemistry Biofilms FECAL MICROBIOTA RNA lcsh:Q Nitrification OXIDIZING BACTERIA |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 6, p e99517 (2014) PLOS ONE |
| ISSN: | 1932-6203 |
| Popis: | The use of mixed microbial communities (microbiomes) for biotechnological applications has steadily increased over the past decades. However, these microbiomes are not readily available from public culture collections, hampering their potential for widespread use. The main reason for this lack of availability is the lack of an effective cryopreservation protocol. Due to this critical need, we evaluated the functionality as well as the community structure of three different types of microbiomes before and after cryopreservation with two cryoprotective agents (CPA). Microbiomes were selected based upon relevance towards applications: (1) a methanotrophic co-culture (MOB), with potential for mitigation of greenhouse gas emissions, environmental pollutants removal and bioplastics production; (2) an oxygen limited autotrophic nitrification/denitrification (OLAND) biofilm, with enhanced economic and ecological benefits for wastewater treatment, and (3) fecal material from a human donor, with potential applications for fecal transplants and pre/probiotics research. After three months of cryopreservation at -80 degrees C, we found that metabolic activity, in terms of the specific activity recovery of MOB, aerobic ammonium oxidizing bacteria (AerAOB) and anaerobic AOB (AnAOB, anammox) in the OLAND mixed culture, resumes sooner when one of our selected CPA [dimethyl sulfoxide (DMSO) and DMSO plus trehalose and tryptic soy broth (DMSO+TT)] was added. However, the activity of the fecal community was not influenced by the CPA addition, although the preservation of the community structure (as determined by 16S rRNA gene sequencing) was enhanced by addition of CPA. In summary, we have evaluated a cryopreservation protocol that succeeded in preserving both community structure and functionality of value-added microbiomes. This will allow individual laboratories and culture collections to boost the use of microbiomes in biotechnological applications. |
| Databáze: | OpenAIRE |
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