Genetic diversity of the root-knot nematodeMeloidogyne ethiopicaand development of a species-specific SCAR marker for its diagnosis

Autor: Marcilene F.A. Santos, Maria Ritta A. Almeida, Valdir R. Correa, Philippe Castagnone-Sereno, R. M. D. G. Carneiro, Vanessa S. Mattos, Myrian S. Tigano
Přispěvatelé: Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Ministério da Agricultura, Pecuária e Abastecimento [Brasil] (MAPA), Governo do Brasil-Governo do Brasil, Departamento de Fitopatologia, University of Brasilia, Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), EMBRAPA-Recursos Geneticos e Biotecnologia, CAPES
Rok vydání: 2013
Předmět:
Zdroj: Plant Pathology
Plant Pathology, Wiley, 2014, 63 (2), pp.476-483. ⟨10.1111/ppa.12108⟩
ISSN: 0032-0862
1365-3059
DOI: 10.1111/ppa.12108
Popis: International audience; Meloidogyne ethiopica is an important nematode pathogen causing serious economic damage to grapevine in Chile. In Brazil, M.ethiopica has been detected with low frequency in kiwifruit and other crops. The objectives of this study were to evaluate the intraspecific genetic variability of M.ethiopica isolates from Brazil and Chile using AFLP and RAPD markers and to develop a species-specific SCAR-PCR assay for its diagnosis. Fourteen isolates were obtained from different geographic regions or host plants. Three isolates of an undescribed Meloidogyne species and one isolate of M.ethiopica from Kenya were included in the analysis. The results showed a low level of diversity among the M.ethiopica isolates, regardless of their geographical distribution or host plant origin. The three isolates of Meloidogyne sp. showed a high homogeneity and clustered separately from M.ethiopica (100% bootstrap). RAPD screenings of M.ethiopica allowed the identification of a differential DNA fragment that was converted into a SCAR marker. Using genomic DNA from pooled nematodes as a template, PCR amplification with primers designed from this species-specific SCAR produced a fragment of 350bp in all 14 isolates of M.ethiopica tested, in contrast with other species tested. This primer pair also allowed successful amplification of DNA from single nematodes, either juveniles or females and when used in multiplex PCR reactions containing mixtures of other root-knot nematode species, thus showing the sensitivity of the assay. Therefore, the method developed here has potential for application in routine diagnostic procedures.
Databáze: OpenAIRE
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