Electrospun silk nanofibers improve differentiation potential of human induced pluripotent stem cells to insulin producing cells
| Autor: | Seyed Ehsan Enderami, Saeid Abediankenari, Hossein Mahboudi, Reyhaneh Nassiri Mansour, Majid Mossahebi-Mohammadi, Seyedeh Fatemeh Ahmadi, Reza Salarinia, Hossein Ranjbaran |
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| Rok vydání: | 2019 |
| Předmět: |
Genetic Markers
Scaffold Materials science Cell Survival Cellular differentiation Induced Pluripotent Stem Cells Cell Culture Techniques Nanofibers Silk Gene Expression Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Biomaterials Cell therapy Tissue culture Islets of Langerhans Tissue engineering Humans Insulin MTT assay Induced pluripotent stem cell Glucose Transporter Type 2 Homeodomain Proteins Tissue Scaffolds Cell Differentiation 021001 nanoscience & nanotechnology 0104 chemical sciences Cell biology Mechanics of Materials Nanofiber Trans-Activators 0210 nano-technology |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 108 |
| ISSN: | 1873-0191 |
| Popis: | With regard to lifestyle and diet, one of the problems that threaten mankind is diabetes. Given the lack of responsiveness to available drug therapies, the advances made in recent decades in tissue engineering and cell therapy have created a great deal of hope for the treatment of this disease. In this study, silk nanofibrous scaffold (3D) was fabricated by electrospinning and then its biocompatibility and non-toxicity by MTT assay. After that, scaffold supportive effects on human induced pluripotent stem cells (hiPSCs) differentiation to insulin producing cells (IPCs) was studied at the gene and protein levels. IPCs related genes and proteins were up regulated in electrospun silk group significantly greater than tissue culture plates group (2D). In addition, another part of the results demonstrated that differentiated cells on 2D and 3D groups have great functional properties including C-peptide and insulin secreting. It can be concluded that silk nanofibers has a great potential for use in pancreatic tissue engineering applications by support viability, growth and IPCs differentiation of iPSCs. |
| Databáze: | OpenAIRE |
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