Candidate reference genes for quantitative gene expression analysis in Lagerstroemia indica
| Autor: | Gao Lulu, Rutong Yang, Peng Wang, Fenni Lv, Manli Chen, Ya Li, Qing Wang |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Candidate gene Eukaryotic Initiation Factor-1 Cold treatment Flowers Sodium Chloride Biology Genes Plant Real-Time Polymerase Chain Reaction Salt Stress Sensitivity and Specificity 03 medical and health sciences 0302 clinical medicine Gene Expression Regulation Plant Stress Physiological Reference genes Arabidopsis Gene expression Genetics Reference gene Molecular Biology Arabidopsis Proteins Cold-Shock Response Gene Expression Profiling fungi food and beverages General Medicine Lagerstroemia indica biology.organism_classification Lagerstroemia Droughts Plant Leaves 030104 developmental biology Organ Specificity 030220 oncology & carcinogenesis Target gene Algorithms Transcription Factors |
| Zdroj: | Molecular Biology Reports. 48:1677-1685 |
| ISSN: | 1573-4978 0301-4851 |
| DOI: | 10.1007/s11033-021-06209-z |
| Popis: | Quantitative gene expression analysis by qPCR requires reference genes for normalization. Lagerstroemia indica (crape myrtle) is a popular ornamental plant in the world, but suitable endogenous reference genes are lacking. To find suitable reference genes, we evaluated the stabilities of nine candidate genes in six experimental data sets: six different tissues, three leaf colors, nine flower colors, and under three abiotic stresses (salt, drought, cold) using four statistical algorithms. A target gene LiMYB56 (homolog of Arabidopsis MYB56) was used to verify the authenticity and accuracy of the candidate reference genes. The results showed that the combination of two stably expressed reference genes, rather than a single reference gene, improved the accuracy of the qPCR. LiEF1α-2 + LiEF1α-3 was best for the tissue, salt treatment, and drought treatment sets; LiEF1α-2 + LiEF1α-1 was optimal for leaf color; LiEF1α-2 + LiACT7 was optimal for cold treatment; and LiUBC + LiEF1α-1 was best for the flower color set. Notably, LiEF1α-2 had high expression stability in all six experimental sets, implying it may be a good reference gene for expression studies in L. indica. Our results will facilitate future gene expression studies in L. indica. |
| Databáze: | OpenAIRE |
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