Ulva intestinalis Protein Extracts Promote In Vitro Collagen and Hyaluronic Acid Production by Human Dermal Fibroblasts

Autor: Thierry Maugard, Amandine Adrien, Pierre-Edouard Bodet, Stanislas Baudouin, Nicolas Bridiau, Justine Bodin, Delphine Dufour
Přispěvatelé: LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), SEPROSYS, Séparations, Procédés, Systèmes, Université de La Rochelle (ULR)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
collagen
Hydrolyzed protein
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Ulva intestinalis
Cell
Pharmaceutical Science
Peptide
seaweed proteins
Polysaccharide
01 natural sciences
Hydrolysate
Article
Analytical Chemistry
lcsh:QD241-441
03 medical and health sciences
chemistry.chemical_compound
lcsh:Organic chemistry
Drug Discovery
Hyaluronic acid
hyaluronic acid
medicine
Physical and Theoretical Chemistry
Fibroblast
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
biology
010604 marine biology & hydrobiology
anti-aging
Organic Chemistry
biology.organism_classification
human dermal fibroblast
3. Good health
medicine.anatomical_structure
chemistry
Biochemistry
Chemistry (miscellaneous)
Molecular Medicine
Zdroj: Molecules
Molecules, MDPI, 2020, 25 (9), pp.2091. ⟨10.3390/molecules25092091⟩
Molecules, Vol 25, Iss 2091, p 2091 (2020)
Volume 25
Issue 9
ISSN: 1420-3049
DOI: 10.3390/molecules25092091⟩
Popis: With the increase in life expectancy, reducing the visible signs of skin aging has become a major issue. A reduction in collagen and hyaluronic acid synthesis by fibroblasts is a feature of skin aging. The green seaweed, Ulva intestinalis, is an abundant and rich source of nutrients, especially proteins and peptides. The aim of this study was to assess the potential cosmetic properties of a protein fraction from Ulva intestinalis (PROT-1) containing 51% of proteins and 22% of polysaccharides, and its enzymatic peptide hydrolysates on human dermal fibroblasts. PROT-1 was extracted using a patented acid- and solvent-free process (FR2998894 (B1)). The biochemical characterization and chromatographic analysis showed a main set of proteins (25 kDa). To demonstrate the anti-aging potential of PROT-1, fibroblast proliferation and collagen and hyaluronic acid production were assessed on fibroblast cell lines from donors aged 20 years (CCD-1059Sk) and 46 years (CCD-1090Sk). PROT-1 induced a significant increase in collagen and hyaluronic acid production per cell, and a reduction in cell proliferation without increasing cell mortality. These effects were reversed after protein hydrolysis of PROT-1, showing the central role of proteins in this promising anti-aging property.
Databáze: OpenAIRE
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