Cloning and Expression of Multiple Cytochrome P450 Genes: Induction by Fipronil in Workers of the Red Imported Fire Ant (Solenopsis invicta Buren)
| Autor: | Xin-nian Zeng, Rukun Cui, Lei Zhang, Xiwu Gao, Bai-zhong Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Insecticides Gene Expression lcsh:Medicine Artificial Gene Amplification and Extension Toxicology Pathology and Laboratory Medicine 01 natural sciences Median lethal dose Biochemistry Polymerase Chain Reaction Red imported fire ant chemistry.chemical_compound Rapid amplification of cDNA ends Cytochrome P-450 Enzyme System Gene expression Medicine and Health Sciences Cloning Molecular lcsh:Science Fipronil Phylogeny Multidisciplinary biology Agriculture Complementary DNA Insects Nucleic acids Insect Pests Agrochemicals Detoxification Sequence Analysis Research Article Fire ant DNA Complementary Arthropoda Forms of DNA Molecular Sequence Data Research and Analysis Methods Gene Expression Regulation Enzymologic Microbiology 03 medical and health sciences Pests Sequence Motif Analysis Botany Genetics Animals Amino Acid Sequence Molecular Biology Techniques Sequencing Techniques Molecular Biology Sequence Homology Amino Acid Ants Lethal dose lcsh:R Organisms Cytochrome P450 Biology and Life Sciences DNA biology.organism_classification Invertebrates Hymenoptera 010602 entomology 030104 developmental biology chemistry biology.protein Pyrazoles lcsh:Q |
| Zdroj: | PLoS ONE, Vol 11, Iss 3, p e0150915 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Both exogenous and endogenous compounds can induce the expression of cytochrome P450 genes. The insect cytochrome P450 genes related to insecticide resistance are likely to be expressed as the "first line of defense" when challenged with insecticides. In this study, four cytochrome P450 genes, SinvCYP6B1, SinvCYP6A1, SinvCYP4C1, and SinvCYP4G15, were firstly isolated from workers of the red imported fire ant (Solenopsis invicta) through rapid amplification of cDNA ends (RACE) and sequenced. The fipronil induction profiles of the four cytochrome P450 genes and the two previously isolated CYP4AB1 and CYP4AB2 were characterized in workers. The results revealed that the expression of SinvCYP6B1, SinvCYP6A1, CYP4AB2, and SinvCYP4G15, increased 1.4-fold and 1.3-fold more than those of acetone control, respectively, after 24 h exposure to fipronil at concentrations of 0.25 μg mL-1 (median lethal dose) and 0.56 μg mL-1 (90% lethal dose), while no significant induction of the expression of CYP4AB1 and SinvCYP4C1 was detected. Among these genes, SinvCYP6B1 was the most significantly induced, and its maximum expression was 3.6-fold higher than that in acetone control. These results might suggest that multiple cytochrome P450 genes are co-up-regulated in workers of the fire ant through induction mechanism when challenged with fipronil. These findings indicated that cytochrome P450 genes play an important role in the detoxification of insecticides and provide a theoretical basis for the mechanisms of insecticide metabolism in the fire ant. |
| Databáze: | OpenAIRE |
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