Effect of DNA-interacting drugs on phage T7 RNA polymerase
| Autor: | D Wilmańska, Kazimierz Studzian, Marek Gniazdowski, Mariola Piestrzeniewicz, G Płucienniczak |
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| Rok vydání: | 1998 |
| Předmět: |
RNA-dependent RNA polymerase
General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Transcription (biology) Bacteriophage T7 RNA polymerase medicine T7 RNA polymerase Polymerase biology Aminoacridines Chemistry Distamycins RNA Netropsin DNA-Directed RNA Polymerases Amides Molecular biology Intercalating Agents Anti-Bacterial Agents Biochemistry Dactinomycin biology.protein DNA medicine.drug |
| Zdroj: | Acta Biochimica Polonica. 45:127-132 |
| ISSN: | 1734-154X 0001-527X |
| DOI: | 10.18388/abp.1998_4295 |
| Popis: | 9-Aminoacridine carboxamide derivatives studied here form with DNA intercalative complexes which differ in the kinetics of dissociation. Inhibition of total RNA synthesis catalyzed by phage T7 and Escherichia coli DNA-dependent RNA polymerases correlates with the formation of slowly dissociating acridine-DNA complex of time constant of 0.4-2.3 s. Their effect on RNA synthesis is compared with other ligands which form with DNA stable complexes of different steric properties. T7 RNA polymerase is more sensitive to distamycin A and netropsin than the E. coli enzyme while less sensitive to actinomycin D. Actinomycin induces terminations in the transcript synthesized by T7 RNA polymerase. Despite low dissociation rates of DNA complexes with acridines and pyrrole antibiotics no drug dependent terminations are observed with these ligands. |
| Databáze: | OpenAIRE |
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