Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage
| Autor: | Amando Flores, Beatriz Mesa-Pereira, Carlos Medina, Eduardo Santero, Eva María Camacho |
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| Přispěvatelé: | Junta de Andalucía, Ministerio de Economía y Competitividad (España) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Salmonella Salicylate Effector Chemistry Therapeutic proteins Antitumoral drugs Cell Heterologous medicine.disease_cause Cell biology Bacterial lysis 03 medical and health sciences Cytosol 030104 developmental biology medicine.anatomical_structure Drug delivery medicine Cytotoxic T cell Protein expression Secretion Intracellular |
| Zdroj: | Digital.CSIC: Repositorio Institucional del CSIC Consejo Superior de Investigaciones Científicas (CSIC) Host-Pathogen Interactions ISBN: 9781493976034 Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Salmonella is probably the intracellular pathogen most extensively studied. Once inside the cell, this bacterium produces different proteins involved in the infection process known as effectors that translocate through its own secretion systems to the eukaryotic cytosol exerting diverse effects on the cell. Additionally, Salmonella can be engineered to include a protein expression system that, upon the addition of an inducer molecule, can produce heterologous proteins at a specific time during the course of the infection. The effect of such proteins on the eukaryotic (i.e., tumoral) cells can be detected following distinct approaches, which converts Salmonella in an effective tool to produce proteins inside eukaryotic cells with different purposes, such as killing tumoral cells. Here, we present diverse technics currently used to produce proteins by Salmonella inside tumoral cells and analyze its cytotoxic effect. This work was supported by the Grant ‘Proyecto de Excelencia P07-CVI02518’ from the Andalusian government and by Spanish Ministry of Science and Innovation grants BIO2014-57545-R. |
| Databáze: | OpenAIRE |
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