Expression profile of host restriction factors in HIV-1 elite controllers
Autor: | Michael David, Xutao Deng, Mohamed Abdel-Mohsen, Rebecca Hoh, Manqing Li, Elizabeth Sinclair, Satish K. Pillai, Joseph K. Wong, Mohamed S. Salama, Teri Liegler, Hussam Ghanem, Douglas F. Nixon, Steven G. Deeks, Rui André Saraiva Raposo |
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Jazyk: | angličtina |
Předmět: |
Intrinsic immunity
CD4-Positive T-Lymphocytes schlafen 11 T cell Viremia HIV Infections Biology HIV Long-Term Survivors 03 medical and health sciences 0302 clinical medicine TRIM Interferon Virology medicine Humans Retroviral restriction factors 030304 developmental biology 0303 health sciences p21 BST2/tetherin T cell activation Research Gene Expression Profiling Nuclear Proteins APOBEC3 medicine.disease ISG15 3. Good health Gene expression profiling medicine.anatomical_structure Elite controllers Infectious Diseases 030220 oncology & carcinogenesis Immunology biology.protein HIV-1 Antibody Viral load Biomarkers medicine.drug |
Zdroj: | Retrovirology Abdel-Mohsen, Mohamed; Raposo, Rui André; Deng, Xutao; Li, Manqing; Liegler, Teri; Sinclair, Elizabeth; et al.(2013). Expression profile of host restriction factors in HIV-1 elite controllers. Retrovirology, 10(1), 106. doi: http://dx.doi.org/10.1186/1742-4690-10-106. Retrieved from: http://www.escholarship.org/uc/item/1sd73537 |
ISSN: | 1742-4690 |
DOI: | 10.1186/1742-4690-10-106 |
Popis: | Background Several host-encoded antiviral factors suppress HIV-1 replication in a cell-autonomous fashion in vitro. The relevance of these defenses to the control of HIV-1 in vivo remains to be elucidated. We hypothesized that cellular restriction of HIV-1 replication plays a significant role in the observed suppression of HIV-1 in "elite controllers", individuals who maintain undetectable levels of viremia in the absence of antiretroviral therapy (ART). We comprehensively compared the expression levels of 34 host restriction factors and cellular activation levels in CD4+ T cells and sorted T cell subsets between elite controllers, HIV-1-infected (untreated) non-controllers, ART-suppressed, and uninfected individuals. Results Expression of schlafen 11, a codon usage-based inhibitor of HIV-1 protein synthesis, was significantly elevated in CD4+ T cells from elite controllers as compared to both non-controllers (p = 0.048) and ART-suppressed individuals (p = 0.024), with this effect most apparent in central memory CD4+ T cells. Schlafen 11 expression levels were comparable between controllers and uninfected individuals. Cumulative restriction factor expression was positively correlated with CD4+ T cell activation (r2 = 0.597, p 2 = 0.34, p = 0.015), and expression of ISG15 (r2 = 0.73, p Conclusions Host restriction factor expression typically scales with cellular activation levels. However, the elevated mRNA and protein expression of schlafen 11, despite low activation and viral load, violates the global pattern and may be a signature characteristic of HIV-1 elite control. |
Databáze: | OpenAIRE |
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