Bone marrow mesenchymal stem cells regulate TGF-β to adjust neuroinflammation in postoperative central inflammatory mice
Autor: | Weidong Mi, Yun‐Feng Li, You-Zhi Zhang, Zhi‐Peng Xv, Zhen‐Zhen Sun |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Lipopolysaccharides Male Lipopolysaccharide medicine.medical_treatment Apoptosis Smad2 Protein Biochemistry chemistry.chemical_compound Mice 0302 clinical medicine Transforming Growth Factor beta Medicine Postoperative Period Saline Neurons Behavior Animal hemic and immune systems Cell Differentiation 030220 oncology & carcinogenesis Signal transduction medicine.symptom Signal Transduction medicine.medical_specialty Cell Survival Inflammation Bone Marrow Cells Enzyme-Linked Immunosorbent Assay Mesenchymal Stem Cell Transplantation 03 medical and health sciences stomatognathic system Internal medicine Animals Cognitive Dysfunction Molecular Biology Neuroinflammation business.industry Mesenchymal Stem Cells Cell Biology medicine.disease Mice Inbred C57BL Disease Models Animal 030104 developmental biology Endocrinology chemistry business Postoperative cognitive dysfunction Transforming growth factor |
Zdroj: | Journal of cellular biochemistry. 121(1) |
ISSN: | 1097-4644 |
Popis: | Background Postoperative cognitive dysfunction (POCD) is one of the common postoperative complications, which is more common in aged patients. POCD mainly manifests as cognitive function changes after surgery, such as memory decline and inattention. In some severe cases, patients may suffer from personality changes and (or) social behavior decline. The aim of the current study is to confirm the effect and elucidate the mechanism of bone marrow mesenchymal stem cells (BMSCs) in postoperative central inflammatory mice. Methods Mice were randomly assigned to four groups: sham, sham+BMSCs, model, and BMSCs group. In the model group, mice were intraperitoneally injected 8 mg/kg per day lipopolysaccharide for 5 days. In sham+BMSCs and BMSCs group, BMSCs (1 × 10 7 ) in 100 µL saline were injected into sham mice and model mice, respectively. Results In the model group, transforming growth factor β (TGF-β) protein expression was significantly increased, compared with that in the sham group. BMSCs were treated into postoperative central inflammatory mice, which resulted in a decreased of TGF-β protein expression. TGF-β and smad2 protein expression were suppressed, and apoptosis rate and inflammation were inhibited in coculture with BMSCs. The suppression of TGF-β inhibited the effects of BMSCs on apoptosis rate and inflammation in postoperative central inflammatory through a smad2 signaling pathway. The promotion of TGF-β reduced the effects of BMSCs on apoptosis rate and inflammation in postoperative central inflammatory through a smad2 signaling pathway. Conclusion The present study demonstrates that BMSCs regulates TGF-β to adjust neuroinflammation in postoperative central inflammatory mice. |
Databáze: | OpenAIRE |
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