Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis
| Autor: | Jorge Enrique Gómez-Marín, Nestor Cardona, Juan Carlos Sepúlveda-Arias, Manuel A. Patarroyo, Laura Taborda, Alejandra de-la-Torre, Elizabeth Torres-Morales |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Lymphocyte Protozoan Proteins Carboxy terminal sequence CD8-Positive T-Lymphocytes Cytokine production T-Lymphocyte Subsets CD8+ T lymphocyte Child Cell proliferation T lymphocyte subpopulation Th1 cell CD8 antigen General Medicine Flow Cytometry SAG1 antigen Parasite virulence Cytokines CD4 antigen Toxoplasma Human Microbiology (medical) Ex vivo study Clinical article Mononuclear Immunology Toxoplasma gondii Antigens Protozoan Human leukocyte antigen Antigen specificity Article Antigen Antigens CD ROP18 protein Humans Parasite antigen Immune response Protein serine threonine kinase CD4+ T lymphocyte Tumor necrosis factor alpha medicine.disease Virology Toxoplasmosis Human cell chemistry CD3 antigen Leukocytes Mononuclear Asymptomatic disease Peptides Immunostimulation IL10 CD4-Positive T-Lymphocytes Unclassified drug Th1 Th2 chemistry.chemical_compound Interleukin 10 Leukocytes Immunology and Allergy Flow cytometry Middle aged Leukocyte antigen Interferon gamma biology ROP18 Virulence factor Middle Aged Protein-Serine-Threonine Kinases CD Chemistry medicine.anatomical_structure Protozoan Female Adult Adolescent Protozoal protein Mononuclear cell Protein Serine-Threonine Kinases Protein p30 Secretion (process) Young Adult Immune system Th2 Cells medicine Antigens Cytokine Gamma interferon Cell Proliferation Th1 Cells Nonhuman biology.organism_classification Th2 cell P30 protein Controlled study Vaccine CD8 |
| Zdroj: | Repositorio EdocUR-U. Rosario Universidad del Rosario instacron:Universidad del Rosario |
| ISSN: | 1432-1831 |
| Popis: | We determined the specific lymphocyte proliferative response and cytokine profile production regarding Toxoplasma P30 (2017 from virulent and non-virulent strain) and ROP18 protein-derived peptides (from clonal lineages I, II and III) in 19 patients having ocular toxoplasmosis, five suffering chronic asymptomatic infection, nine with congenital toxoplasmosis and eight Toxoplasma negative people. A Beckman Coulter FC500 flow cytometer was used for determining antigen-specific T cells (CD3+ CD4+ or CD3+ CD8+ cells) in peripheral blood culture. IFN ? and IL10 levels were determined in culture supernatants. Specific CD4+ and CD8+ T cell response to total antigen and P30- and ROP18-derived peptides was observed in infected people. Ocular toxoplasmosis patients had a preferential Th2 response after antigenic stimulation. Non-virulent peptide 2017 was able to shift response toward Th1 in congenitally infected children and virulent peptide 2017 induced a Th2 response in chronically infected, asymptomatic people. An immune response in human toxoplasmosis after ex vivo antigenic stimulation was Th1- or Th2-skewed, depending on a patient’s clinical condition. Colombian ocular toxoplasmosis patients’ immune response was Th2-skewed, regardless of the nature of antigen stimulus. © 2014, Springer-Verlag Berlin Heidelberg. |
| Databáze: | OpenAIRE |
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