Utilization of phage display to identify antigenic regions in the PCV2 capsid protein for the evaluation of serological responses in mice and pigs
| Autor: | Fabiana de Almeida Araújo Santos, Gustavo Costa Bressan, Abelardo Silva-Júnior, Juliana Lopes Rangel Fietto, Márcia Rogéria de Almeida, Carlos Ueira-Veira, Viviane Sisdelli Assao, Zélia Inês Portela Lobato, Luíz Ricardo Goulart, Ana Paula Carneiro, Rafael Locatelli Salgado, Marcus Rebouças Santos |
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| Rok vydání: | 2018 |
| Předmět: |
Circovirus
0301 basic medicine Phage display Swine Identify antigenic viruses animal diseases Enzyme-Linked Immunosorbent Assay Biology Antibodies Viral Sensitivity and Specificity Virus Epitope Epitopes Mice 03 medical and health sciences Blood serum Antigen Neutralization Tests Virology Animals PCV2 capsid protein Circoviridae Infections Swine Diseases Evaluation of serological Immunogenicity virus diseases Viral Vaccines General Medicine biology.organism_classification Antibodies Neutralizing Porcine circovirus 030104 developmental biology Capsid Capsid Proteins Cell Surface Display Techniques Peptides Mice and pigs |
| Zdroj: | LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/s00705-018-3816-8 |
| Popis: | Porcine circovirus 2 (PCV2) is associated with a series of swine diseases. There is a great interest in improving our understanding of the immunology of PCV2, especially the properties of the viral capsid protein Cap-PCV2 and how they relate to the immunogenicity of the virus and the subsequent development of vaccines. Phage display screening has been widely used to study binding affinities for target proteins. The aim of this study was to use phage display screening to identify antigenic peptides in the PCV2 capsid protein. After the selection of peptides, five of them presented similarity to sequences found in cap-PCV2, and four peptides were synthesized and used for immunization in mice: 51–CTFGYTIKRTVT-62 (PS14), 127-CDNFVTKATALTY-138 (PS34), 164-CKPVLDSTIDY-173 (PC12), and 79-CFLPPGGGSNT-88 (PF1). Inoculation with the PC12 peptide led to the highest production of antibodies. Furthermore, we used the PC12 peptide as an antigen to examine the humoral response of swine serum by ELISA. The sensitivity and specificity of this assay was 88.9% and 92.85%, respectively. Altogether, characterization of immunogenic epitopes in the capsid protein of PCV2 may contribute to the improvement of vaccines and diagnostics. |
| Databáze: | OpenAIRE |
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