A Novel pAA Virulence Plasmid Encoding Toxins and Two Distinct Variants of the Fimbriae of Enteroaggregative Escherichia coli
Autor: | Betina Hebbelstrup Jensen, Carsten Struve, Camilla Adler Sørensen, Erik J. Boll, Håvard Jenssen, Rie Jønsson, Nadia Boisen, Karen A. Krogfelt, Flemming Scheutz, Katrine Grimstrup Joensen |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Genetics Microbiology (medical) biology 030106 microbiology Fimbria virulence genes diarrhea Virulence biochemical phenomena metabolism and nutrition Microbiology fimbriae 03 medical and health sciences EAEC 030104 developmental biology Plasmid Intestinal mucosa Pilin Enteroaggregative Escherichia coli biology.protein Gene Pathogen enteroaggregative E. coli Original Research |
Zdroj: | Frontiers in Microbiology |
ISSN: | 1664-302X |
Popis: | Enteroaggregative Escherichia coli (EAEC) is an increasingly recognized pathogen associated with acute and persistent diarrhea worldwide. While EAEC strains are considered highly heterogeneous, aggregative adherence fimbriae (AAFs) are thought to play a pivotal role in pathogenicity by facilitating adherence to the intestinal mucosa. In this study, we optimized an existing multiplex PCR to target all known AAF variants, which are distinguished by differences in their pilin subunits. We applied the assay on a collection of 162 clinical Danish EAEC strains and interestingly found six, by SNP analysis phylogenetically distinct, strains harboring the major pilin subunits from both AAF/III and AAF/V. Whole-genome and plasmid sequencing revealed that in these six strains the agg3A and agg5A genes were located on a novel pAA plasmid variant. Moreover, the plasmid also encoded several other virulence genes including some not previously found on pAA plasmids. Thus, this plasmid endows the host strains with a remarkably high number of EAEC associated virulence genes hereby likely promoting strain pathogenicity. |
Databáze: | OpenAIRE |
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