Medaka vasa gene has an exonic enhancer for germline expression
Autor: | Haobing Zhao, Junling Zhang, Mingyou Li, Jing Wei, Yunhan Hong |
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Rok vydání: | 2014 |
Předmět: |
Fish Proteins
Transcription Genetic Somatic cell Amino Acid Motifs Molecular Sequence Data Oryzias Enhancer RNAs E-box Biology Transfection Germline DEAD-box RNA Helicases Exon Genes Reporter Sequence Homology Nucleic Acid Gene expression Genetics Animals Transgenes Enhancer Promoter Regions Genetic Binding Sites Base Sequence vasa gene General Medicine Exons Molecular biology Enhancer Elements Genetic Gene Expression Regulation Gene Deletion Plasmids |
Zdroj: | Gene. 555(2) |
ISSN: | 1879-0038 |
Popis: | Enhancers are DNA sequences that enhance gene transcription in a position- and orientation-independent manner. Many enhancers controlling somatic gene expression have been described. Enhancers controlling germline expression have remained rare. Here we report the identification of V35 as a first exonic germline enhancer in vertebrates. V35 constitutes the first 35bp of exon 1 of the medaka vasa gene. V35 is required for vasa promoter function and sufficient to increase transcriptional activity of a heterologous promoter by ~13 fold in either forward or reverse orientation. V35 contains CAGCAGCACGAG for two paired E box-like motifs. Upon incubation with nuclear extract from spermatogonial cells, V35 formed three DNA-protein complexes. We show that complex formation is inhibited partially by oligos containing an E box or E box-like motif but completely by V35 and oligos that contain overlapped E box and E box-like motifs. Most importantly, V35 is sufficient to drive transgene expression in germ cells of developing embryos. These results establish V35 as the first exonic germline enhancer in a lower vertebrate, and provide evidence for the importance of exonic sequences in controlling germ gene expression. |
Databáze: | OpenAIRE |
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