Mating type determination within a microsatellite multiplex for the fungal pathogen Pseudogymnoascus destructans, the causative agent of white-nose disease in bats
| Autor: | Nicola M. Fischer, Marcus Fritze, Jeffrey T. Foster, Sébastien J. Puechmaille, Andrea Altewischer, Kevin P. Drees, Serena E. Dool |
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| Přispěvatelé: | Ernst-Moritz-Arndt-Universität Greifswald, Northern Arizona University [Flagstaff], Universität Greifswald - University of Greifswald, Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226 |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mating type Pseudogymnoascus food.ingredient Population [SDV.BDLR.RA]Life Sciences [q-bio]/Reproductive Biology/Asexual reproduction Asexual reproduction Biology 010603 evolutionary biology 01 natural sciences [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction 03 medical and health sciences Geomyces food Pseudogymnoascus destructans Genetics Mating education ComputingMilieux_MISCELLANEOUS Ecology Evolution Behavior and Systematics Mating type determination education.field_of_study biology.organism_classification 3. Good health 030104 developmental biology [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis |
| Zdroj: | Conservation Genetics Resources Conservation Genetics Resources, Springer, 2018, ⟨10.1007/s12686-018-1064-6⟩ |
| ISSN: | 1877-7260 1877-7252 |
| Popis: | Emerging infectious diseases are a severe conservation threat for a variety of plants and animals. In North America, several bat species are threatened by white-nose disease, which has caused an unprecedented mass mortality of > 6 million bats since 2006. The fungus Pseudogymnoascus (Geomyces) destructans is the causative agent of the disease. Though asexual reproduction is the norm, sexual reproduction is possible as two mating types exist. Sexual reproduction has been implicated in the emergence of virulent strains of fungi but to date no rapid means of mating type characterisation was available for P. destructans. In this study, three mating type-specific primer sets were designed and tested on 80 isolates. The primers were multiplexed with microsatellite loci allowing for rapid and concurrent genotyping and mating type assignment. These markers will have great utility in better understanding and predicting the population dynamics and evolutionary potential of this fungus, including the emergence of virulent strains. |
| Databáze: | OpenAIRE |
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