Two-photon microscopy imaging of oxidative stress in human living erythrocytes
| Autor: | Vasili Tsakanov, Gohar Tsakanova, Elina Arakelova, S. G. Haroutiunian, Anna Ayvazyan, Yeva B. Dalyan, Arsen Arakelyan, Violetta Ayvazyan, Rouben Aroutiounian, Stepan Tatikyan |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Antioxidant Chemistry medicine.medical_treatment Cell analysis medicine.disease_cause Article Atomic and Molecular Physics and Optics In vitro model 03 medical and health sciences 030104 developmental biology Two-photon excitation microscopy Microscopy medicine Biophysics Fluorescence microscope Oxidative stress Laser beams Biotechnology |
| Zdroj: | Biomedical Optics Express. 8:5834 |
| ISSN: | 2156-7085 |
| Popis: | Red blood cells (RBCs) are known to be the most suitable cells to study oxidative stress, which is implicated in the etiopathology of many human diseases. The goal of the current study was to develop a new effective approach for assessing oxidative stress in human living RBCs using two-photon microscopy. To mimic oxidative stress in human living RBCs, an in vitro model was generated followed by two-photon microscopy imaging. The results revealed that oxidative stress is clearly visible on the two-photon microscopy images of RBCs under oxidative stress compared to no fluorescence in controls (P |
| Databáze: | OpenAIRE |
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