A difference in the incorporation of 14C into hippurate glycine from dl -[2-14C]glutamate and dl -[5-14C]glutamate in guinea pigs
| Autor: | Tahsin A. J. Ali, Kathleen V. Rowsell, E V Rowsell |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
History
Glycine biosynthesis Transamination Stereochemistry Guinea Pigs Glycine Glyoxylate cycle Metabolism in Whole Organisms Isotope dilution Benzoates Education Glutamates Animals Carbon Radioisotopes Amino Acids Pyruvates Bond cleavage chemistry.chemical_classification Chemistry Hippurates Glutamate receptor Glyoxylates Succinates Computer Science Applications Biochemistry Ketoglutaric Acids |
| Zdroj: | Biochemical Journal. 152:357-363 |
| ISSN: | 0306-3283 |
| DOI: | 10.1042/bj1520357 |
| Popis: | The specific radioactivity of urinary hippurate glycine was determined after injecting guinea pigs with benzoate and either dl-[2-14C]glutamate or dl-[5-14C]glutamate. The isotope dilution factor for the formation of [14C]glycine was significantly greater (30%) with C-2 labelled glutamate. With either form of labelled glutamate the hippurate glycine was largely carboxyl-group labelled. The observations suggest a route for the incorporation of glutamate carbon into glycine that involves C-5 but not C-2. A hypothesis for glycine biosynthesis from l-glutamate is advanced, consistent with these findings, that includes conversion of l-glutamate to 4-hydroxy-2-oxoglutarate, the scission of the latter to glyoxylate and pyruvate, and the formation of glycine by transamination. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|