Cytotoxic and apoptogenic effect of hypericin, the bioactive component of Hypericum perforatum on the MCF-7 human breast cancer cell line
| Autor: | Hossein Ghasemzadeh Kolagar, Soheila Yadollah-Damavandi, Mohammad Amin Azizi, Mohammad Amin Javidi, Tina Parsa, Ehsan Jangholi, Yekta Parsa, Seyed Abbas Mirmalek, Reza Alizadeh-Navaei, Seyed Alireza Salimi-Tabatabaee |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Cancer Research
Cytotoxic medicine.medical_treatment Apoptosis Hypericin Pharmacology 030218 nuclear medicine & medical imaging 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Breast cancer Breast Cancer Hypericum perforatum Genetics Medicine Cytotoxic T cell skin and connective tissue diseases Chemotherapy business.industry medicine.disease MCF-7 chemistry Oncology 030220 oncology & carcinogenesis Adenocarcinoma business Primary Research |
| Zdroj: | Cancer Cell International |
| ISSN: | 1475-2867 |
| DOI: | 10.1186/s12935-016-0279-4 |
| Popis: | Background Breast cancer is the most prevalent malignancies among the women that have a high mortality. Previous studies demonstrated that hypericin, a bioactive component of Hypericum perforatum have a cytotoxic effect on the malignant cell lines. However, an anti-carcinogenic activity of hypericin on MCF-7 is uncertain. To investigate the cytotoxic effect of hypericin on MCF-7 cells, a human breast adenocarcinoma cell-line, that resistance to chemotherapy. Methods The MCF-7 and fibroblast (as normal cell line) were treated with various concentrations of hypericin, and Cisplatin as a positive control for 24 and 48 h. Cytotoxicity activity was measured and confirmed by MTT assay and Trypan blue staining, respectively. In addition, Apoptosis were determined by Annexin V/Propidium Iodide assay. Immunocytochemistry (ICC) analysis for bcl2 and p53 proteins performed to further investigate different expression of these genes in different samples. Results Both cisplatin and the hypericin exhibited a dose-dependent cytotoxic effect in the MCF-7 cell line. Although the LD50 of the hypericin was significantly lower when compared to cispaltin (5 vs. 20 μg/ml), it continued to decrease the growth rate of the MCF-7 cells when tested at higher concentration than LD50. In contrast, cisplatine, at higher concentration than LD50, completely inhibited the growth of the MCF-7 in 48 h. Regarding Annexin V/Propidium results, treatment of MCF-7 cells with LD50 concentration of cisplatin and hypericin showed 60 and 52 % apoptosis in 24 h, respectively. ICC analysis for bcl2 and p53 also confirmed our results; in treated samples for the dose of LD50 in 24 and 48 h of cisplatin and hypercin, more cells expressed p53 (guardian of cells in front of tumor formation/progression) and less expressed bcl2 (which has anti apoptotic activity) compared to untreated samples. Conclusions Considering that hypericin showed to be cytotoxic, it seems to be a chemopreventive agent and a good candidate for antineoplastic drug development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|