Association of Anti-F(ab′)2 Antibodies (Pepsin Agglutinators) with Immune Complexes as Determined by Enzyme-Linked Immunosorbent Assays
| Autor: | Michael P. Davey, Leonhard Korngold |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
chemistry.chemical_classification
biology Serial dilution Complement Activating Enzymes Complement C1q Immunology Enzyme-Linked Immunosorbent Assay Antigen-Antibody Complex General Medicine Molecular biology Antibodies Anti-Idiotypic Immunoglobulin Fab Fragments Immune system Enzyme Pepsin chemistry Rheumatoid Factor biology.protein Humans Immunology and Allergy Antibody |
| Zdroj: | International Archives of Allergy and Immunology. 67:278-283 |
| ISSN: | 1423-0097 1018-2438 |
| DOI: | 10.1159/000233031 |
| Popis: | A solid-phase assay for the detection of anti-F(ab′)2 antibodies is described. Wells of microtiter plates are coated with F(ab′)2, dilutions of sera are added, and IgG bound to the solid phase is detected using peroxidase-labeled Protein A. Anti-F(ab′)2 antibodies were found in 61% of sera from patients with rheumatoid arthritis, 77% with subacute bacterial endocarditis, and 34% with systemic lupus erythematosus. Simultaneous analysis of these sera for immune complexes (IC), using modified Clq and monoclonal rheumatoid factor assays, showed that a correlation existed between anti-F(ab′)2 antibodies and the levels of IC. Characterization of anti-F(ab′)2 antibodies by inhibition and absorption experiments and by immunological and physical means indicated that they were similar to serum proteins described in the 1960s and designated pepsin agglutinators. |
| Databáze: | OpenAIRE |
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