Detecting changes in the caenorhabditis elegans intestinal environment using an engineered bacterial biosensor
| Autor: | Geraint M.H. Thomas, Filipe Cabreiro, Evgeniy R. Galimov, Leonor M. Quintaneiro, Luca Rosa, Chris P. Barnes, Jack W. Rutter, Tanel Ozdemir |
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| Přispěvatelé: | Wellcome Trust |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
E. coli Nissle 1917 ved/biology.organism_classification_rank.species Biomedical Engineering Computational biology Gut flora 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) 03 medical and health sciences Synthetic biology In vivo 010608 biotechnology Model organism Caenorhabditis elegans microbiome engineering Organism 030304 developmental biology 0303 health sciences biology ved/biology Rational design General Medicine biology.organism_classification biosensors In vitro synthetic biology |
| Popis: | Caenorhabditis elegans has become a key model organism within biology. In particular, the transparent gut, rapid growing time, and ability to create a defined gut microbiota make it an ideal candidate organism for understanding and engineering the host microbiota. Here we present the development of an experimental model that can be used to characterize whole-cell bacterial biosensors in vivo. A dual-plasmid sensor system responding to isopropyl β-d-1-thiogalactopyranoside was developed and fully characterized in vitro. Subsequently, we show that the sensor was capable of detecting and reporting on changes in the intestinal environment of C. elegans after introducing an exogenous inducer into the environment. The protocols presented here may be used to aid the rational design of engineered bacterial circuits, primarily for diagnostic applications. In addition, the model system may serve to reduce the use of current animal models and aid in the exploration of complex questions within general nematode and host-microbe biology. |
| Databáze: | OpenAIRE |
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