Osteogenic differentiation of bone marrow mesenchymal stem cells by adenovirus-mediated expression of leptin
| Autor: | Yuhui Zhang, Jianmin Liu, Jing Yingying, Lai-Xing Wang, Jinchuan Liang, Zhi-Jian Yue, Guosheng Han, Xiaowu Hu |
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| Rok vydání: | 2010 |
| Předmět: |
Leptin
medicine.medical_specialty Physiology Recombinant Fusion Proteins Clinical Biochemistry Genetic Vectors Osteocalcin Adipokine Core Binding Factor Alpha 1 Subunit Biology Biochemistry Core Binding Factor beta Subunit Adenoviridae Cellular and Molecular Neuroscience Endocrinology Internal medicine medicine Humans Cells Cultured Cell Proliferation digestive oral and skin physiology Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Transfection Alkaline Phosphatase Cell biology Up-Regulation medicine.anatomical_structure biology.protein Alkaline phosphatase Bone marrow Stem cell Genetic Engineering hormones hormone substitutes and hormone antagonists |
| Zdroj: | Regulatory peptides. 163(1-3) |
| ISSN: | 1873-1686 |
| Popis: | Previous studies demonstrate that leptin has an osteogenic differentiation effect on bone marrow mesenchymal stem cells (MSCs). However, the effect of adenovirus-mediated leptin on MSCs differentiation has not been reported. To explore the mechanism, we constructed a recombinant adenoviral vector Ad-leptin and transfected propagated MSCs in vitro. The effects of Ad-leptin on MSCs growth and osteogenic differentiation were examined. The results showed that Ad-leptin inhibited the transfected MSCs growth significantly, and up-regulated osteocalcin expression and alkaline phosphatase activity. The expression of Cbfalpha1 and Cbfbeta which were the key factors in osteogenic differentiation was also up-regulated. All the findings suggest that genetic engineering of MSCs to express leptin gene may have potential application in the treatment of several genetic diseases and bone reconstruction. |
| Databáze: | OpenAIRE |
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