EPSPSgene amplification conferring resistance to glyphosate in windmill grass (Chloris truncata)in Australia
| Autor: | The D Ngo, Jenna M Malone, Peter Boutsalis, Gurjeet Gill, Christopher Preston |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Pesticide resistance Population Glycine Chromosomal translocation Poaceae 01 natural sciences chemistry.chemical_compound Gene duplication Botany education Gene Plant Proteins Chloris truncata education.field_of_study biology Herbicides Australia Gene Amplification 04 agricultural and veterinary sciences General Medicine Pesticide biology.organism_classification chemistry Insect Science Glyphosate 040103 agronomy & agriculture 0401 agriculture forestry and fisheries 3-Phosphoshikimate 1-Carboxyvinyltransferase Agronomy and Crop Science Herbicide Resistance 010606 plant biology & botany |
| Zdroj: | Pest Management Science. 74:1101-1108 |
| ISSN: | 1526-498X |
| DOI: | 10.1002/ps.4573 |
| Popis: | Background Five glyphosate-resistant populations of Chloris truncata originally collected from New South Wales were compared with one susceptible (S) population from South Australia to confirm glyphosate resistance and elucidate possible mechanisms of resistance. Results Based on the amounts of glyphosate required to kill 50% of treated plants (LD50 ), glyphosate resistance (GR) was confirmed in five populations of C. truncata (A536, A528, T27, A534 and A535.1). GR plants were 2.4-8.7-fold more resistant and accumulated less shikimate after glyphosate treatment than S plants. There was no difference in glyphosate absorption and translocation between GR and S plants. The EPSPS gene did not contain any point mutation that had previously been associated with resistance to glyphosate. The resistant plants (A528 and A536) contained up to 32-48 more copies of the EPSPS gene than the susceptible plants. Conclusion This study has identified EPSPS gene amplification contributing to glyphosate resistance in C. truncata. In addition, a Glu-91-Ala mutation within EPSPS was identified that may contribute to glyphosate resistance in this species. © 2017 Society of Chemical Industry. |
| Databáze: | OpenAIRE |
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