Spatial Distribution and Initial Changes of SSEA-1 and Other Cell Adhesion-related Molecules on Mouse Embryonic Stem Cells Before and During Differentiation
| Autor: | Fengming Yue, Kohei Johkura, Yasumitsu Okouchi, Kazuhiko Asanuma, Li Cui, Katsunori Sasaki, Naoko Ogiwara |
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| Rok vydání: | 2004 |
| Předmět: |
0301 basic medicine
Histology Cellular differentiation Cell Retinoic acid Lewis X Antigen Tretinoin Biology Tetraspanin 29 Article Flow cytometry Mice 03 medical and health sciences chemistry.chemical_compound Microscopy Electron Transmission Antigens CD Cell Adhesion medicine Animals Cell adhesion Cells Cultured Membrane Glycoproteins 030102 biochemistry & molecular biology medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Stem Cells Cell Differentiation Cell sorting Embryo Mammalian Flow Cytometry Intercellular Adhesion Molecule-1 Molecular biology Embryonic stem cell Cell biology Platelet Endothelial Cell Adhesion Molecule-1 030104 developmental biology medicine.anatomical_structure chemistry embryonic structures Microscopy Electron Scanning Anatomy Stem cell |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 52:1447-1457 |
| ISSN: | 1551-5044 0022-1554 |
| Popis: | We examined the distribution of cell adhesion-related molecules (CAMs) among mouse embryonic stem (ES) cells and the spatial distribution on cell surfaces before and during differentiation. The cell-cell heterogeneity of SSEA-1, PECAM-1, and ICAM-1 among the undifferentiated cells in the ES cell colonies was evident by immunohistochemistry and immuno-SEM, supporting the flow cytometry findings. In contrast, most undifferentiated ES cells strongly expressed CD9. SSEA-1 was located preferentially on the edge of low protuberances and microvilli and formed clusters or linear arrays of 3–20 particles. PECAM-1 and ICAM-1 were randomly localized on the free cell surfaces, whereas CD9 was preferentially localized on the microvilli or protuberances, especially in the cell periphery. Both the SSEA-1+ fraction and the SSEA-1− fraction of magnetic cell sorting (MACS) formed undifferentiated colonies after plating. Flow cytometry showed that these populations reverted separately again to a culture with a mixed phenotype. Differentiation induced by retinoic acid downregulated the expression of all CAMs. Immuno-SEM showed decreases of SSEA-1 in the differentiated ES cells, although some clustering still remained. Our findings help to elucidate the significance of these molecules in ES cell maintenance and differentiation and suggest that cell surface antigens may be useful for defining the phenotype of undifferentiated and differentiated ES cells. |
| Databáze: | OpenAIRE |
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