Assessment of Cellular Uptake Efficiency According to Multiple Inhibitors of Fe3O4-Au Core-Shell Nanoparticles: Possibility to Control Specific Endocytosis in Colorectal Cancer Cells
| Autor: | Yu Jin Kim, Bo Gi Park, Young Keun Kim, Sang Hwan Nam, Nam Hyuk Cho, Kyu Back Lee, Taek-Chin Cheong, Ji Hyun Min |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Phagocytosis media_common.quotation_subject Muc1 Vimentin 02 engineering and technology 010402 general chemistry Endocytosis 01 natural sciences chemistry.chemical_compound lcsh:TA401-492 General Materials Science Internalization MUC1 Cytochalasin D media_common biology Cancer targeting Receptor-mediated endocytosis Fe3O4-Au core-shell NPs 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences chemistry Drug delivery Biophysics biology.protein lcsh:Materials of engineering and construction. Mechanics of materials 0210 nano-technology |
| Zdroj: | Nanoscale Research Letters, Vol 15, Iss 1, Pp 1-10 (2020) |
| ISSN: | 1556-276X |
| Popis: | Magnetite (Fe3O4)-gold (Au) core-shell nanoparticles (NPs) have unique magnetic and optical properties. When combined with biological moieties, these NPs can offer new strategies for biomedical applications, such as drug delivery and cancer targeting. Here, we present an effective method for the controllable cellular uptake of magnetic core-shell NP systems combined with biological moieties. Vimentin, which is the structural protein, has been biochemically confirmed to affect phagocytosis potently. In addition, vimentin affects exogenic materials internalization into cells even though under multiple inhibitions of biological moieties. In this study, we demonstrate the cellular internalization performance of Fe3O4-Au core-shell NPs with surface modification using a combination of biological moieties. The photofluorescence of vimentin-tagged NPs remained unaffected under multiple inhibition tests, indicating that the NPs were minimally influenced by nystatin, dynasore, cytochalasin D, and even the Muc1 antibody (Ab). Consequently, this result indicates that the Muc1 Ab can target specific molecules and can control specific endocytosis. Besides, we show the possibility of controlling specific endocytosis in colorectal cancer cells. |
| Databáze: | OpenAIRE |
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