Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis
| Autor: | Jan Hansmann, Emmanuela M. Anandarajah, Klaus D. Erttmann, Guido Schramm, Dominic Winter, Norbert W. Brattig, Dana Ditgen, Eva Liebau |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
animal structures Article Subject Trichuris suis Cellular homeostasis Chemotaxis Biology biology.organism_classification In vitro Microbiology lcsh:Infectious and parasitic diseases 03 medical and health sciences 030104 developmental biology Infectious Diseases Intestinal mucosa Parasitology lcsh:RC109-216 Thioredoxin Wound healing Strongyloides ratti Research Article |
| Zdroj: | Journal of Parasitology Research, Vol 2016 (2016) Journal of Parasitology Research |
| ISSN: | 2090-0031 2090-0023 |
| Popis: | The cellular redox state is important for the regulation of multiple functions and is essential for the maintenance of cellular homeostasis and antioxidant defense. In the excretory/secretory (E/S) products ofStrongyloides rattiandTrichuris suissequences for thioredoxin (Trx) and Trx-like protein (Trx-lp) were identified. To characterize the antioxidant Trx-lp and its interaction with the parasite’s mucosal habitat,S. rattiandT. suisTrx-lps were cloned and recombinantly expressed. The primary antioxidative activity was assured by reduction of insulin and IgM. Further analysis applying anin vitromucosal 3D-cell culture model revealed that the secreted Trx-lps were able to bind to monocytic and intestinal epithelial cells and induce the time-dependent release of cytokines such as TNF-α, IL-22, and TSLP. In addition, the redox proteins also possessed chemotactic activity for monocytic THP-1 cells and fostered epithelial wound healing activity. These results confirm that the parasite-secreted Trx-lps are multifunctional proteins that can affect the host intestinal mucosa. |
| Databáze: | OpenAIRE |
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