Genomic analysis of oral Campylobacter concisus strains identified a potential bacterial molecular marker associated with active Crohn's disease
| Autor: | Susan J. Connor, Li Zhang, Rupert W. Leong, Fang Liu, Michael Grimm, Sophie Octavia, Mark M. Tanaka, Chin Yen Alfred Tay, Ruiting Lan, Stephen M. Riordan, Heung Kit Leslie Chung, Rena Ma |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Genetic Markers Male medicine.medical_specialty Adolescent Epidemiology 030106 microbiology Immunology Campylobacter concisus Enterotoxin Microbiology Inflammatory bowel disease Article 03 medical and health sciences Young Adult Medical microbiology Plasmid Bacterial Proteins Crohn Disease Virology Drug Discovery Campylobacter Infections medicine Humans Symbiosis Aged Crohn's disease Mouth biology Whole Genome Sequencing Campylobacter General Medicine Genomics Chromosomes Bacterial Middle Aged medicine.disease biology.organism_classification Ulcerative colitis Infectious Diseases Child Preschool biology.protein Parasitology Female Antibody Genome Bacterial |
| Zdroj: | Emerging Microbes & Infections |
| ISSN: | 2222-1751 |
| Popis: | Campylobacter concisus is an oral bacterium that is associated with inflammatory bowel disease (IBD) including Crohn’s disease (CD) and ulcerative colitis (UC). C. concisus consists of two genomospecies (GS) and diverse strains. This study aimed to identify molecular markers to differentiate commensal and IBD-associated C. concisus strains. The genomes of 63 oral C. concisus strains isolated from patients with IBD and healthy controls were examined, of which 38 genomes were sequenced in this study. We identified a novel secreted enterotoxin B homologue, Csep1. The csep1 gene was found in 56% of GS2 C. concisus strains, presented in the plasmid pICON or the chromosome. A six-nucleotide insertion at the position 654–659 bp in csep1 (csep1-6bpi) was found. The presence of csep1-6bpi in oral C. concisus strains isolated from patients with active CD (47%, 7/15) was significantly higher than that in strains from healthy controls (0/29, P = 0.0002), and the prevalence of csep1-6bpi positive C. concisus strains was significantly higher in patients with active CD (67%, 4/6) as compared to healthy controls (0/23, P = 0.0006). Proteomics analysis detected the Csep1 protein. A csep1 gene hot spot in the chromosome of different C. concisus strains was found. The pICON plasmid was only found in GS2 strains isolated from the two relapsed CD patients with small bowel complications. This study reports a C. concisus molecular marker (csep1-6bpi) that is associated with active CD. |
| Databáze: | OpenAIRE |
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