Exosomes populate the cerebrospinal fluid of preterm infants with post-haemorrhagic hydrocephalus

Autor: Andriana Gialeli, Bryony McPherson, Axel Heep, Robert Spaull, Aled Clayton, James B. Uney, Oscar Cordero-Llana
Rok vydání: 2018
Předmět:
Zdroj: Spaull, R, McPherson, B, Gialeli, A, Clayton, A, Uney, J, Heep, A & Cordero-Llana, Ó 2019, ' Exosomes populate the cerebrospinal fluid of preterm infants with post-haemorrhagic hydrocephalus ', International Journal of Developmental Neuroscience, vol. 73, pp. 59-65 . https://doi.org/10.1016/j.ijdevneu.2019.01.004
ISSN: 1873-474X
0736-5748
Popis: Background\ud\udPreterm infants are at risk of germinal matrix haemorrhage-intraventricular haemorrhage (GMH-IVH) which leads to post-haemorrhagic hydrocephalus (PHH) in 30% of infants; this is associated with moderate-severe neurodevelopmental impairment and confers significant risk of cerebral palsy. There are however no predictive indicators of the severity or long-term outcome after GMH-IVH. In recent years, endosome-derived extracellular vesicles (EVs) or exosomes have been isolated from biofluids and shown to mediate intercellular communication via selective enrichment in proteins and micro-RNAs.\ud\ud\udMethods\ud\udThis study aimed to isolate and characterise EVs from the cerebrospinal fluid (CSF) of 3 preterm infants with PHH using nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) with immunogold protein labelling, and micro-RNA analysis.\ud\ud\udResults\ud\udNTA of unaltered CSF revealed a heterogeneous and dynamic population of EVs. Exosomal-sized EVs were isolated by differential ultracentrifugation and TEM confirmed the presence of CD63+ and CD81+ exosomes. The micro-RNAs miR-9, miR-17, miR-26a, miR-124 and miR-1911 were detected within the exosome-enriched fraction and profiled over time.\ud\ud\udConclusion\ud\udThis is the first reported characterisation of exosomes from the CSF of preterm infants with post-haemorrhagic hydrocephalus.
Databáze: OpenAIRE
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