Equine Amniotic Microvesicles and Their Anti-Inflammatory Potential in a Tenocyte Model In Vitro
| Autor: | Fausto Cremonesi, Giovanni Camussi, Ornella Parolini, C. Perrini, Bruna De Vita, Bruna Corradetti, Maria Chiara Deregibus, Maria Giovanna Marini, Luisa Pascucci, Pietro Romele, Davide Bizzaro, riccardo tasquier, Anna Lange-Consiglio |
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| Přispěvatelé: | Universita degli Studi di Milano, University of Torino, University of Perugia, Universita Politecnica Delle Marche, Universidade Estadual Paulista (Unesp), Istituto Ospedaliero |
| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
musculoskeletal diseases 0301 basic medicine Gene Expression regenerative medicine Inflammation Amniotic Stem Cells Gene Expression Stress Biology Stress Peripheral blood mononuclear cell Amniotic Stem Cells Proinflammatory cytokine Tendons 03 medical and health sciences 0302 clinical medicine Cell-Derived Microparticles In vivo medicine Settore BIO/13 - BIOLOGIA APPLICATA Animals Amnion Collagenases Horses Cells Cultured Cell Proliferation tendon cells Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology Hematology Microvesicles In vitro Cell biology Tenocytes 030104 developmental biology inflammation Culture Media Conditioned 030220 oncology & carcinogenesis Immunology amnion microvesicles inflammation tendon cells regenerative medicine Leukocytes Mononuclear Tumor necrosis factor alpha medicine.symptom microvesicles Developmental Biology |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1557-8534 1547-3287 |
| DOI: | 10.1089/scd.2015.0348 |
| Popis: | Made available in DSpace on 2018-12-11T17:02:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-04-15 Administration of horse amniotic mesenchymal cells (AMCs) and their conditioned medium (AMC-CM) improves the in vivo recovery of spontaneous equine tendon lesions and inhibits in vitro proliferation of peripheral blood mononuclear cells (PBMC). This process may involve microvesicles (MVs) as an integral component of cell-to-cell communication during tissue regeneration. In this study, the presence and type of MVs secreted by AMCs were investigated and the response of equine tendon cells to MVs was studied using a dose-response curve at different concentrations and times. Moreover, the ability of MVs to counteract in vitro inflammation of tendon cells induced by lipopolysaccharide was studied through the expression of some proinflammatory genes such as metallopeptidase (MPP) 1, 9, and 13 and tumor necrosis factor-α (TNFα), and expression of transforming growth factor-β (TGF-β). Lastly, the immunomodulatory potential of MVs was investigated. Results show that AMCs secrete MVs ranging in size from 100 to 200 nm. An inverse relationship between concentration and time was found in their uptake by tendon cells: the maximal uptake occurred after 72 h at a concentration of 40 × 106 MVs/mL. MVs induced a downregulation of MMP1, MMP9, MMP13, and TNFα expression without affecting PBMC proliferation, contrary to CM and supernatant. Our data suggest that MVs contribute to in vivo healing of tendon lesions, alongside soluble factors in AMC-CM. Large Animal Hospital Reproduction Unit Universita degli Studi di Milano, Via dell'Universita 6 Department of Internal Medicine Molecular Biotechnology Center University of Torino Department of Veterinary Medicine University of Perugia Department of Biochemistry Biology and Genetics Universita Politecnica Delle Marche Department of Animal Reproduction and Radiology FMVZ UNESP Centro di Ricerca E. Menni Fondazione Poliambulanza Istituto Ospedaliero Department of Veterinary Medical Science Universita degli Studi di Milano Department of Animal Reproduction and Radiology FMVZ UNESP |
| Databáze: | OpenAIRE |
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